Mitogen-Activated Protein Kinase 14 (MAPK14) (pThr180) antibody

Antigen: Mitogen-Activated Protein Kinase 14 (MAPK14)

Synonyms: RK, p38, EXIP, Mxi2, CSBP1, CSBP2, CSPB1, PRKM14, PRKM15, SAPK2A, p38ALPHA, Hog, CRK1, CSBP, Exip, Csbp1, Csbp2, Prkm14, Prkm15, Sapk2A, p38Hog, p38alpha, MGC105413, p38b, zp38b, MAPK14, MGC142910, 186F5S, BG:DS00797.3, D-p38, D-p38b, Dm p38b, Dp38, ESTS:186F5S, Mpk34C, anon-sts23, p38 MAPK, p38 beta, p38B, p38beta, DmelCG7393, CG7393

Epitope: pThr180

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Dot Blot (Dot), Immunohistochemistry (IHC)

Catalog no.: ABIN361512

Additional Information

Alternative name: Phospho-Thr180/Tyr182 p38 MAPK

Format: Affinity-purified

Description: The three Mitogen-Activated Protein Kinases (MAPKs) are evolutionarily conserved protein kinases that control a vast array of cellular processes. p38 MAPK is one of these kinases and it is activated by both inflammatory cytokines and by stress (Johnson and Lapadat, 2002, Shi and Gaestel, 2002). The p38 MAPK is thought to be particularly important in diseases like asthma and autoimmunity but it also plays important roles in the stress response of the nervous system (Philip and Armstead, 2003, Ying et al., 2002). Like the other MAPKs, p38 is activated by a dual specificity kinase that phosphorylates Thr180 and Tyr182 (Lin et al., 1995). Anti-Phospho Thr180/Tyr182 p38 MAPK Western blot of HeLa cell lysates that had been treated with UV or untreated (Control) showing specific immunolabeling of the ~39k p38 MAPK protein phosphorylated at Thr180 and Tyr182 .

Specificity: Specific for the ~39k p38 MAPK protein phosphorylated at Thr180/Tyr182 in Western blots of C-6 glioma cells prepared with anisomycin treatment. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. The antibody has been directly testet for reactivity in Western blot with human tissue. It is anticipated that the antibody will also react with bovine, canine, chicken, mouse, non-human primates, rat and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Synonyms: RK, p38, EXIP, Mxi2, CSBP1, CSBP2, CSPB1, PRKM14, PRKM15, SAPK2A, p38ALPHA, Hog, CRK1, CSBP, Exip, Csbp1, Csbp2, Prkm14, Prkm15, Sapk2A, p38Hog, p38alpha, MGC105413, p38b, zp38b, MAPK14, MGC142910, 186F5S, BG:DS00797.3, D-p38, D-p38b, Dm p38b, Dp38, ESTS:186F5S, Mpk34C, anon-sts23, p38 MAPK, p38 beta, p38B, p38beta, DmelCG7393, CG7393

Application Details

Application Notes: DB: 1:1000. WB: 1:1000. IHC: 1:250-1:500.

Purification: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Buffer: 10mM HEPES (ph 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10 mini Western blots.

Storage: For long term storage -20°C is recommended. Stable at -20°C for at least 1 year.

Restrictions: For Research Use only

Images

Western blots of HeLa cell lysates that had been treated with UV or untreated (Control) showing specific immunolabeling of the ~39k p38 MAPK protein phosphorylated at Thr180 and Tyr182 .