Superoxide Dismutase 1, Soluble (SOD1) antibody

Antigen: Superoxide Dismutase 1, Soluble (SOD1)

Synonyms: ALS, SOD, ALS1, IPOA, homodimer, Cu, Zn Sod, Zn-SOD, Cu-Zn SOD, Cu/Zn SOD, Cu/Zn superoxide dismutase, Cu/ZnSOD, CuSOD, CuZn SOD, CuZn-SOD, CuZnSOD, Cu[2+]/Zn[2+]SOD, G, SOD-1, SOD1, To, To-1, cSOD, l(3)108, l(3)68Af', l(3)G, DmelCG11793, CG11793, LOC692639, CU/ZN-SOD, sod1, SODC, DKFZP469M1833

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Guinea Pig, Mouse (Murine), Cow (Bovine), Monkey, Coral, Dog (Canine), Hamster, Pig (Porcine), Rabbit, Sheep (Ovine), Xenopus laevis

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN361646

Additional Information

Characteristics: Detects a ~23kDa (human) and 19kDa (other species) proteins corresponding to the molecular mass of Cu/Zn superoxide dismutase (SOD) on SDS PAGE immunoblots.

Alternative name: Cu/Zn SOD

Gene ID: NP_000445.1, 6647

Immunogen: Human Cu/Zn SOD

Description: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extracellular space(9). SOD3 can also be distinguished by its heparin-binding capacity (1).
Synonyms: Superoxide dismutase1, ALS1, IPOA, SOD1, SOD2, SODC

Specificity: Detects 23kDa (human) and 19kDa (other species) proteins, corresponding to the molecular mass of Cu/Zn superoxide dismutase (SOD) on SDS-PAGE immunoblots. Species cross-reactivity: Human, Rat, Mouse, Bovine, Monkey, Coral, Dog, Hamster, Pig, Rabbit, Sheep, Xenopus.

Synonyms: ALS, SOD, ALS1, IPOA, homodimer, Cu, Zn Sod, Zn-SOD, Cu-Zn SOD, Cu/Zn SOD, Cu/Zn superoxide dismutase, Cu/ZnSOD, CuSOD, CuZn SOD, CuZn-SOD, CuZnSOD, Cu[2+]/Zn[2+]SOD, G, SOD-1, SOD1, To, To-1, cSOD, l(3)108, l(3)68Af', l(3)G, DmelCG11793, CG11793, LOC692639, CU/ZN-SOD, sod1, SODC, DKFZP469M1833

Application Details

Application Notes: 0.2ug/ml was sufficient for detection of Cu/Zn SOD in 20ug of HeLa cell lysate

Concentration: 1mg/mL

Buffer: PBS pH7.0, 50% glycerol, 0.09% sodium azide

Storage: -20 °C

Storage Shipping Temp Max: Blue Ice or 4 °C

Research Area: Oxidative Stress

Restrictions: For Research Use only

Images

CuZn SOD (human), human placenta. Courtesy of Joan Telfer, University of Glasgow