TLR4 antibody

Antigen: TLR4

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine)

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blotting (WB)

Catalog no.: ABIN361724

Additional Information

Characteristics: Detects ~75-80kDa when tested against partial recombinant mouse TLR4 (extra-cellular portion plus His-tag).

Gene ID: NP_612564.1, 7099

Immunogen: Developed against a synthetic peptide corresponding to amino acids 420-435 of human TLR4.

Description: The Toll-like receptor (TLR) family in mammal comprises a family of trans-membrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and 1L-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylo-genetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Amount this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediatiors of LPS signaling (4,5). The mouse TLR4 cDNA codes for a protein consisting of 839 amino acids, with an approximate molecular weight of 90kDa (6).
Synonyms: ARMD10, CD284, TLR 4, TOLL, Toll like receptor 4

Specificity: Detects ~75-80kDa when tested against partial recombinant mouse TLR4 (extracellular portion plus His-tag) Species Cross-reactivity Human, Mouse

Application Details

Application Notes: 1-3ug/mL for Western blot, 1:50 for IHC

Concentration: 1mg/mL

Buffer: PBS, 0.05% BSA, 0.05% sodium azide

Storage: -20 °C

Storage Shipping Temp Max: Blue Ice or 4 °C

Research Area: Cell Signaling

Restrictions: For Research Use only

Images

TLR4-MD-2 recomb, 95kD

TLR4, mouse colitis colon, Amplifier

TLR4, mouse spleen tissue

Publications

Publications: Medzhitov, Preston-Hurlburt, Janeway: "A human homologue of the Drosophila Toll protein signals activation of adaptive immunity." in: Nature, Vol. 388, Issue 6640, pp. 394-7, 1997 (PubMed).

Medzhitov, Janeway: "Innate immunity: the virtues of a nonclonal system of recognition." in: Cell, Vol. 91, Issue 3, pp. 295-8, 1997 (PubMed).

Muzio, Natoli, Saccani et al.: "The human toll signaling pathway: divergence of nuclear factor kappaB and JNK/SAPK activation upstream of tumor necrosis factor receptor-associated factor 6 (TRAF6)." in: The Journal of experimental medicine, Vol. 187, Issue 12, pp. 2097-101, 1998 (PubMed).

Takeuchi, Hoshino, Kawai et al.: "Differential roles of TLR2 and TLR4 in recognition of gram-negative and gram-positive bacterial cell wall components." in: Immunity, Vol. 11, Issue 4, pp. 443-51, 1999 (PubMed).

Poltorak, Ricciardi-Castagnoli, Citterio et al.: "Physical contact between lipopolysaccharide and toll-like receptor 4 revealed by genetic complementation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 5, pp. 2163-7, 2000 (PubMed).

Chuang, Ulevitch: "Identification of hTLR10: a novel human Toll-like receptor preferentially expressed in immune cells." in: Biochimica et biophysica acta, Vol. 1518, Issue 1-2, pp. 157-61, 2001 (PubMed).