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LAMP2 antibody (Lysosomal-Associated Membrane Protein 2)

Details for Product anti-LAMP2 Antibody No. ABIN361746, Supplier: Log in to see
Antigen
  • cd107b
  • CD107b
  • DKFZp459J126
  • Lamp-2
  • LAMP-2
  • lamp-2
  • Lamp-2a
  • Lamp-2b
  • Lamp-2c
  • LAMP2
  • lamp2
  • lampb
  • LAMPB
  • LGP-B
  • LGP110
  • Mac3
Reactivity
Human, Mouse (Murine), Rabbit
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176
33
23
19
6
Host
Rat
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120
116
1
Clonality (Clone)
Monoclonal ()
Conjugate
This LAMP2 antibody is un-conjugated
53
37
27
23
10
10
6
5
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
195
155
97
89
76
73
68
37
36
11
9
8
4
4
2
2
2
2
1
1
1
1
Supplier
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Immunogen Purified preparation of mouse liver lysosomal membranes
Clone GL2A7
Specificity Detects ~100-110 kDa.
Purification Protein G Purified
Alternative Name LAMP2 (LAMP2 Antibody Abstract)
Background Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures, with 37 % sequence homology (2). They are both transmembrane glycoproteins that are localized primarily in lysosomes and late endosomes. Newly synthesized molecules are mostly transported from the trans-Golgi network directly to endosomes and then to lysosomes. A second pathway involves the lamps being delivered from the Golgi to the cell surface, and then along the endocytic pathway to the lysosomes. A minor pathway involves transport via the plasma membrane (3). LAMP2 has also been detected at the plasma membrane of cells, as well as in cells that secrete lysosomal hydrolases. A study in the developmental expresses patterns of membrane LAMP2 transcripts indicate a possible involvement of this protein in cell-cell or cell-extracellular matrix interaction, and appear to reflect tissue and cell type specific roles of lysosomes during morphogenesis (4). Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxyl-terminal tyrosine based motif (YXXI) (5). This stimulation has also been shown to have an associated release of histamine, leukotriene C 9$) and prostaglandin D 9@), which shows that LAMP1 and LAMP2 are activation markers for normal mast cells (5). They have also been linked to the inflammatory response in that they promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and therefore possibly the adhesion of PBMC to the site of inflammation (6). LAMP2 has also been shown to be critical for autophagy, in conversion of early autophagic vacuoles to vacuoles which rapidly degrade their content (7).
Cellular Localization: Cell Membrane | Endosome | Lysosome | Endosome membrane | Lysosome membrane
Gene ID 16784
NCBI Accession NP_001017959
UniProt P17047
Research Area Stem Cells, Hematopoietic Progenitors, Autophagy, Metabolism, Organelles
Application Notes Recommended Dilution: WB (1:1000), ICC/IF (1:500), optimal dilutions for assays should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
 image for anti-LAMP2 antibody (Lysosomal-Associated Membrane Protein 2) (ABIN361746) LAMP2 (GL2A7), IF showing distribution of lysosomes in CEC Courtesy of Eunduck E P K...
Background publications Grützkau, Smorodchenko, Lippert, Kirchhof, Artuc, Henz: "LAMP-1 and LAMP-2, but not LAMP-3, are reliable markers for activation-induced secretion of human mast cells." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 61, Issue 1, pp. 62-8, 2004

Tanaka, Guhde, Suter, Eskelinen, Hartmann, Lüllmann-Rauch, Janssen, Blanz, von Figura, Saftig: "Accumulation of autophagic vacuoles and cardiomyopathy in LAMP-2-deficient mice." in: Nature, Vol. 406, Issue 6798, pp. 902-6, 2000

Lichter-Konecki, Moter, Krawisz, Schlotter, Hipke, Konecki: "Expression patterns of murine lysosome-associated membrane protein 2 (Lamp-2) transcripts during morphogenesis." in: Differentiation; research in biological diversity, Vol. 65, Issue 1, pp. 43-58, 1999

Hoening, Sandoval, von Figura: "A di-leucine-based motif in the cytoplasmic tail of LIMP-II and tyrosinase mediates selective binding of AP-3." in: The EMBO journal, Vol. 17, Issue 5, pp. 1304-14, 1998

Rohrer, Schweizer, Russell, Kornfeld: "The targeting of Lamp1 to lysosomes is dependent on the spacing of its cytoplasmic tail tyrosine sorting motif relative to the membrane." in: The Journal of cell biology, Vol. 132, Issue 4, pp. 565-76, 1996

Kannan, Stewart, Bounds, Carlsson, Fukuda, Betzing, Holcombe et al.: "Lysosome-associated membrane proteins h-LAMP1 (CD107a) and h-LAMP2 (CD107b) are activation-dependent cell surface glycoproteins in human peripheral blood mononuclear cells which mediate cell adhesion ..." in: Cellular immunology, Vol. 171, Issue 1, pp. 10-9, 1996

Granger, Green, Gabel, Howe, Mellman, Helenius: "Characterization and cloning of lgp110, a lysosomal membrane glycoprotein from mouse and rat cells." in: The Journal of biological chemistry, Vol. 265, Issue 20, pp. 12036-43, 1990