Calnexin-CT antibody

Antigen: Calnexin-CT

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Monkey, Mouse (Murine), Rat (Rattus), Cow (Bovine), Chicken, Dog (Canine), Guinea Pig, Hamster, Pig (Porcine), Quail, Rabbit, Sheep (Ovine), Fruit Fly (Drosophila melanogaster), Xenopus laevis

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Catalog no.: ABIN361782

Additional Information

Gene ID: 403908

UniProt: P24643

Immunogen: Dog Calnexin C-terminal sythetic peptide conjugated to KLH. Identical to human, mouse and rat calnexin sequences over these residues.

Description: Calnexin, an abundant ~90kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90. It consists of a large 50kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail. Unlike its ER counterparts which have a KDEL sequence on their C-terminus to ensure ER retention, calnexin has positively charged cytosolic residues that do the same thing. Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into mulitsubunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their monoglycosylated glycans. Calnexin has also been shown to associate with the major histocompatability complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin.
Synonyms: CANX, CNX, IP90, P90

Characteristics: Accession Number: NP_001003232.1

Specificity: Detects ~90kDa.

Application Details

Application Notes: 1:2000 (WB)

Concentration: 1mg/mL

Purification: Protein A Purified

Buffer: PBS pH7.2, 50% glycerol

Preservative: 0.09% sodium azide

Storage: Store at -20° C. Shipping Temperature: Blue Ice or 4° C

Storage Shipping Temp Max: Blue Ice or 4 °C

Research Area: Cell Signaling, Organelles

Restrictions: For Research Use only

Images

Calnexin CT, rat tissue mix.

Publications

Tjoelker, Seyfried, Eddy et al.: "Human, mouse, and rat calnexin cDNA cloning: identification of potential calcium binding motifs and gene localization to human chromosome 5." in: Biochemistry, Vol. 33, Issue 11, pp. 3229-36, 1994 (PubMed).

Rajagopalan, Xu, Brenner: "Retention of unassembled components of integral membrane proteins by calnexin." in: Science (New York, N.Y.), Vol. 263, Issue 5145, pp. 387-90, 1994 (PubMed).