Calnexin antibody (C-Term)

Catalog No. ABIN361827

Product Details anti-Calnexin Antibody

Target: Calnexin (CANX)

Binding Specificity: C-Term

Reactivity: Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calnexin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Detects the C-terminal domain of Calnexin ~90 kDa. Weak detection in Chicken, Drosophila, and Xenopus tissues

Cross-Reactivity: Chicken, Cow, Dog, Drosophila melanogaster, Guinea Pig, Hamster, Human, Monkey, Mouse, Pig, Quail, Rabbit, Rat, Sheep, Xenopus laevis

Purification: Peptide Affinity Purified

Immunogen: Dog calnexin C-terminal synthetic peptide conjugated to KLH. Identical to human, mouse and rat calnexin sequences over these residues.

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:100)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN361826 was sufficient for detection of Calnexin in 10 μg of HeLa cell lysate by ECL immunoblot analysis.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

References for anti-Calnexin antibody (ABIN361827)

Quintas, Pérez-Núñez, Sánchez, Nogal, Hentze, Castelló, Revilla: "Characterization of the African Swine Fever Virus Decapping Enzyme during Infection." in: Journal of virology, Vol. 91, Issue 24, (2017) (PubMed).

Hernández-Tiedra, Fabriàs, Dávila, Salanueva, Casas, Montes, Antón, García-Taboada, Salazar-Roa, Lorente, Nylandsted, Armstrong, López-Valero, McKee, Serrano-Puebla, García-López, González-Martínez et al.: "Dihydroceramide accumulation mediates cytotoxic autophagy of cancer cells via autolysosome destabilization. ..." in: Autophagy, Vol. 12, Issue 11, pp. 2213-2229, (2017) (PubMed).

Gordo-Gilart, Hierro, Andueza, Muñoz-Bartolo, López, Díaz, Jara, Álvarez: "Heterozygous ABCB4 mutations in children with cholestatic liver disease." in: Liver international : official journal of the International Association for the Study of the Liver, (2015) (PubMed).

Gordo-Gilart, Andueza, Hierro, Martínez-Fernández, DAgostino, Jara, Alvarez: "Functional analysis of ABCB4 mutations relates clinical outcomes of progressive familial intrahepatic cholestasis type 3 to the degree of MDR3 floppase activity." in: Gut, Vol. 64, Issue 1, pp. 147-55, (2014) (PubMed).

Williams, Tulke, Ilegems, Berggren, Broberger: "Expression of nucleobindin 1 (NUCB1) in pancreatic islets and other endocrine tissues." in: Cell and tissue research, Vol. 358, Issue 2, pp. 331-42, (2014) (PubMed).

Martín-Acebes, Merino-Ramos, Blázquez, Casas, Escribano-Romero, Sobrino, Saiz: "The composition of West Nile virus lipid envelope unveils a role of sphingolipid metabolism in flavivirus biogenesis." in: Journal of virology, Vol. 88, Issue 20, pp. 12041-54, (2014) (PubMed).

Target Details for Calnexin

Target: Calnexin (CANX)

Alternative Name: Calnexin (CANX Products)

Synonyms: CNX antibody, IP90 antibody, P90 antibody, 1110069N15Rik antibody, AI988026 antibody, Cnx antibody, D11Ertd153e antibody, canx antibody, clgn antibody, clnx antibody, cnx antibody, PP90 antibody, zgc:63524 antibody, wu:fe06b12 antibody, CANX antibody, calnexin antibody, CalNeXin antibody, calnexin L homeolog antibody, CANX antibody, Canx antibody, cnx-1 antibody, canx.L antibody, canx antibody, LOC100283947 antibody

Background: Calnexin, an abundant ~90 kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50 kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their C-terminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multi-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their mono-glycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatibility complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).

Gene ID: 403908

NCBI Accession: NP_001003232

UniProt: P24643

Pathways: MAPK Signaling, Thyroid Hormone Synthesis