Mitogen-Activated Protein Kinase 3 (MAPK3) antibody

Antigen: Mitogen-Activated Protein Kinase 3 (MAPK3)

Synonyms: ERK1, PRKM3, P44ERK1, P44MAPK, HS44KDAP, HUMKER1A, MGC20180

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus), Cow (Bovine), Sheep (Ovine), Chicken, Fruit Fly (Drosophila melanogaster), Xenopus laevis

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Catalog no.: ABIN361833

Additional Information

Alternative name: Erk1/2

Immunogen: A 35 residue synthetic peptide, corresponding to Erk1 MAP kinase with the CGG spacer group added and the peptide coupled to KLH.

Format: Antiserum

Description: The extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called p44 and p42 MAP kinases, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. Because the 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade, the two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase (1). They are both located in the cytosol and mitochondria (2). While the role of cytosol ERK1/2 is well studied and involved in multiple cellular functions (2), the role of mitochondrial ERK1/2 remains poorly understood. Both ERK 1 and 2 are activated by MEK1 or MEK2, by dual phosphorylation of a threonine and tyrosine residue in the activation loop (TEY motif) (1, 3). Either phosphorylation alone can induce an electrophoretic mobility shift, but both are required for activation of the kinase. This dual phosphorylation is efficiently detected by phosphorylation state-specific antibody directed to the pTEpY motif. Once activated, MAP kinases phosphorylate a broad spectrum of substrates, including cytoskeletal proteins, translation regulators, transcription factors, and the Rsk family of protein kinases (4). ERK1/2 activation is generally thought to confer a survival advantage to cells (5), however there is increasing evidence that suggests that the activation of ERK1/2 also contributes to cell death under certain conditions (5). ERK1/2 also is activated in neuronal and renal epithelial cells upon exposure to oxidative stress and toxicants or deprivation of growth factors, and inhibition of the ERK pathway blocks apoptosis (5).

Specificity: Detects ~44Kda and ~42kDa bands corresponding to the molecular weights of Erk1 and Erk2. Species cross-reactivity: Human, Mouse, Rat, Cow, Sheep, Chicken, Drosophila, Xenopus.

Application Details

Application Notes: Working Dilution WB 1:5000 (ECL) IHC 1:50 Flow cytometry 10ug/mL

Buffer: Whole Rabbit Serum

Storage: -20°C, 1 year+, shipped on cold packs or ambient

Research Area: Signaling, Inflammation

Restrictions: For Research Use only

Images

Erk1-2, Mouse backskin