C-JUN antibody (pThr239)

Catalog No. ABIN361919

The Rabbit Polyclonal anti-C-JUN antibody has been validated for WB, IHC and IF. It is suitable to detect C-JUN in samples from Human, Mouse and Rat. There is 1 publication available.

Quick Overview for C-JUN antibody (pThr239) (ABIN361919)

Target: C-JUN (JUN) (Jun Proto-Oncogene (JUN))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This C-JUN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-C-JUN Antibody

Binding Specificity: pThr239

Specificity: The antibody detects endogenous level of c-Jun only when phosphorylated at threonine239.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed bychromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pThr239 (G-E-T (p) -P-P) derived from Human c-Jun. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-C-JUN antibody (ABIN361919)

Yamamura, Kawakami, Hirata, Ueno, Saini, Majid, Dahiya: "Oncogenic functions of secreted Frizzled-related protein 2 in human renal cancer." in: Molecular cancer therapeutics, Vol. 9, Issue 6, pp. 1680-7, (2010) (PubMed).

Target Details for C-JUN

Target: C-JUN (JUN) (Jun Proto-Oncogene (JUN))

Alternative Name: c-Jun

Background: Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.

Molecular Weight: 43 kDa

NCBI Accession: NP_002219

UniProt: P05412

Pathways: MAPK Signaling, RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling, S100 Proteins