JunB antibody (pSer259)

Catalog No. ABIN361925

There is 1 publication for this product available. The Rabbit Polyclonal anti-JunB antibody is suitable to detect JunB in samples from Human, Mouse and Rat. It has been validated for WB and IHC.

Quick Overview for JunB antibody (pSer259) (ABIN361925)

Target: JunB (JUNB) (Jun B Proto-Oncogene (JUNB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JunB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-JunB Antibody

Binding Specificity: pSer259

Specificity: The antibody detects endogenous level of JunB only when phosphorylated at serine 259.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pSer259 (P-V-S (p) -P-I) derived from Human JunB. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-JunB antibody (ABIN361925)

Vartanian, Masri, Martin, Cloninger, Holmes, Artinian, Funk, Ruegg, Gera: "AP-1 regulates cyclin D1 and c-MYC transcription in an AKT-dependent manner in response to mTOR inhibition: role of AIP4/Itch-mediated JUNB degradation." in: Molecular cancer research : MCR, Vol. 9, Issue 1, pp. 115-30, (2011) (PubMed).

Target Details for JunB

Target: JunB (JUNB) (Jun B Proto-Oncogene (JUNB))

Alternative Name: JunB

Background: Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'.

Molecular Weight: 43 kDa

NCBI Accession: NP_002220

UniProt: P17275