Checkpoint Kinase 2 (CHEK2) (pSer516) antibody

Details for Product No. ABIN362141
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Antigen
Synonyms CHK-2, CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53, hCds1, Cds1, HUCDS1, Rad53, Chk2, fa66f08, wu:fa66f08, zgc:55865
Epitope
pSer516
(36), (35), (28), (28), (16), (13), (13), (11), (9), (7), (6), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(332), (146), (145), (55), (37), (36), (12), (7), (6), (1)
Host
Rabbit
(272), (65), (1), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(257), (109), (89), (60), (58), (42), (31), (28), (9), (4), (1), (1)
Pubmed 3 references available
Quantity 50 µL
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Catalog No. ABIN362141
158.40 $
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Immunogen Peptide sequence around phosphorylation site of pSer516 (Q-P-S (p) -T-S) derived from Human Chk2. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of Chk2 only when phosphorylated at serine 516.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name Chk2
Background In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by Chk2 gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.
Molecular Weight 62 kDa
NCBI Accession NP_001005
UniProt O96017
Research Area Cancer, Cell Cycle, DNA/RNA, Kinases/Phosphatases
Application Notes Western blotting: 1:500-1:1000  
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Lou, Minter-Dykhouse, Wu et al.: "MDC1 is coupled to activated CHK2 in mammalian DNA damage response pathways." in: Nature, Vol. 421, Issue 6926, pp. 957-61, 2003 (PubMed).

Schwarz, Lovly, Piwnica-Worms: "Regulation of the Chk2 protein kinase by oligomerization-mediated cis- and trans-phosphorylation." in: Molecular cancer research : MCR, Vol. 1, Issue 8, pp. 598-609, 2003 (PubMed).

Wu, Chen: "Autophosphorylation of checkpoint kinase 2 at serine 516 is required for radiation-induced apoptosis." in: The Journal of biological chemistry, Vol. 278, Issue 38, pp. 36163-8, 2003 (PubMed).

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