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Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) (pSer1177) antibody

Antigen

Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3)

Synonyms NOS, INOS, NOS2A, HEP-NOS, iNOS, Nos-2, Nos2a, i-NOS, NOS-II, eNOS, Nos-3, ecNOS, 2310065A03Rik, iNos, cNOS, EC-NOS, NOSIII, NOS3, NOS2, nos2, ECNOS
Epitope
Alternatives

pSer1177

Clonality Polyclonal
Host
Alternatives

Rabbit

Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)

Application
Alternatives Immunohistochemistry (IHC), Immunofluorescence (IF)
Catalog no. ABIN362157
Quantity 50µg  (50µg/50µl)  (Variants)
Price 280.00 $   Plus shipping costs $35.00
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Additional Information

Alternative name eNOS (Phospho-Ser1177)
Swiss-Prot P29474
Immunogen The antiserum was produced against synthesized phosphopeptide derived from humaneNOS around the phosphorylation site of serine 1177 (T-Q-S P -F-S).
Isotype IgG  (Matching secondary antibodies)
Specificity ENOS (Phospho-Ser1177) (Human) antibody detects endogenous levels of eNOS only whenphosphorylated at serine 1177.

Application Details

Application Notes IHC: 1:50~1:100 IF:1:100~1:200
Concentration 50µg/50µl
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Buffer Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C. Avoid repeat freeze-thaw cycles.
Restrictions For Research Use only

Alternatives

Alternatives for antigen "Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3)", type "Antibodies"
Hosts Rabbit (47), Mouse (10)
Reactivities Human (46), Rat (Rattus) (32), Mouse (Murine) (29), Cow (Bovine) (3), Pig (Porcine) (2), Dog (Canine) (1), Horse (Equine) (1), Monkey (1), Sheep (Ovine) (1)
Applications Western Blotting (WB) (34), Immunofluorescence (IF) (27), Enzyme Immunoassay (EIA) (12), Immunohistochemistry (IHC) (9), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (9), ELISA (7), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), ELISA (Detection) (1), Flow Cytometry (FACS) (1), Immunoprecipitation (IP) (1)
Epitopes pSer1177 (7), Ab-1176 (3), pSer1176 (3), Ab-1179 (1), Center (1), pSer632 (1)