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zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr319) antibody

Details for Product No. ABIN362163
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Antigen
Synonyms zap70, MGC80670, MGC88962, ZAP70, 70kDa, AI327364, Srk, TZK, ZAP-70, mrtle, mur, SRK, STCD, STD
Epitope
pTyr319
(40), (38), (26), (19), (19), (14), (11), (10), (7), (7), (7), (7), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(335), (159), (111), (49), (48), (37), (24), (24), (1)
Host
Rabbit
(239), (85), (8), (4), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(17), (14), (11), (9), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2)
Application
Western Blotting (WB), Immunofluorescence (IF)
(209), (108), (96), (59), (50), (40), (27), (21), (17), (3), (2), (1)
Pubmed 6 references available
Quantity 50 µL
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Catalog No. ABIN362163
198.00 $
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Immunogen Peptide sequence around phosphorylation site of pTyr319 (S-P-Y (p) -S-D) derived from Human Zap-70. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of VEGFR2 only when phosphorylated at tyrosine 1214.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name Zap-70
Background Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.
Molecular Weight 70 kDa
NCBI Accession NP_001070
UniProt P43403
Research Area Signaling, Tags/Labels, Immunology, Cancer
Application Notes Western blotting: 1:500-1:1000
Immunofluorescence: 1:100-1:200
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Zhao, Weiss: "Enhancement of lymphocyte responsiveness by a gain-of-function mutation of ZAP-70." in: Molecular and cellular biology, Vol. 16, Issue 12, pp. 6765-74, 1997 (PubMed).

Williams, Irvin, Sutor et al.: "Phosphorylation of Tyr319 in ZAP-70 is required for T-cell antigen receptor-dependent phospholipase C-gamma1 and Ras activation." in: The EMBO journal, Vol. 18, Issue 7, pp. 1832-44, 1999 (PubMed).

Tang, Sawasdikosol, Chang et al.: "SLAP, a dimeric adapter protein, plays a functional role in T cell receptor signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 17, pp. 9775-80, 1999 (PubMed).

Ku, Yablonski, Manser et al.: "A PAK1-PIX-PKL complex is activated by the T-cell receptor independent of Nck, Slp-76 and LAT." in: The EMBO journal, Vol. 20, Issue 3, pp. 457-65, 2001 (PubMed).

Salomon, Ficarro, Brill et al.: "Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 2, pp. 443-8, 2003 (PubMed).

Adjali, Marodon, Steinberg et al.: "In vivo correction of ZAP-70 immunodeficiency by intrathymic gene transfer." in: The Journal of clinical investigation, Vol. 115, Issue 8, pp. 2287-95, 2005 (PubMed).

Validation Images
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