zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr493) antibody

Details for Product No. ABIN362166
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Antigen
Synonyms SRK, STCD, STD, TZK, ZAP-70, Srk, zap70, MGC80670, MGC88962, ZAP70
Epitope
pTyr493
(38), (36), (19), (18), (15), (14), (9), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(309), (156), (111), (49), (48), (37), (24), (24)
Host
Rabbit
(210), (88), (8), (4), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(15), (11), (9), (6), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2)
Application
Immunohistochemistry (IHC)
(186), (95), (76), (59), (45), (40), (25), (18), (18), (3), (2), (2), (1)
Pubmed 3 references available
Catalog no. ABIN362166
Quantity 100 µL
Price
286.00 $   Plus shipping costs $45.00
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Peptide sequence around phosphorylation site of pTyr493 (S-Y-Y (p) -T-A) derived from Human Zap-70. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of Zap-70 only when phosphorylated at tyrosine 493.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name Zap-70
Background Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.
Molecular Weight 70 kDa
UniProt P43403
Research Area Signaling, Tags/Labels, Immunology, Cancer
Application Notes Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Chan, Dalton, Johnson et al.: "Activation of ZAP-70 kinase activity by phosphorylation of tyrosine 493 is required for lymphocyte antigen receptor function." in: The EMBO journal, Vol. 14, Issue 11, pp. 2499-508, 1995 (PubMed).

Kong, Dalton, Bubeck Wardenburg et al.: "Distinct tyrosine phosphorylation sites in ZAP-70 mediate activation and negative regulation of antigen receptor function." in: Molecular and cellular biology, Vol. 16, Issue 9, pp. 5026-35, 1996 (PubMed).

Salomon, Ficarro, Brill et al.: "Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 2, pp. 443-8, 2003 (PubMed).

Hosts (210), (88), (8), (4), (1)
Reactivities (309), (156), (111), (49), (48), (37), (24), (24)
Applications (186), (95), (76), (59), (45), (40), (25), (18), (18), (3), (2), (2), (1)
Conjugates (15), (11), (9), (6), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2)
Epitopes (38), (36), (19), (18), (15), (14), (9), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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