Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC) (pThr2609) antibody

Details for Product No. ABIN362178
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Antigen
Synonyms PRKDC, AI326420, AU019811, DNA-PKcs, DNAPDcs, DNAPK, DNPK1, DOXNPH, HYRC1, XRCC7, dxnph, p460, scid, slip, HYRC, p350, dna-pkcs, dnapk, dnpk1, hyrc, hyrc1, prkdc-A, xrcc7, DNA-PKCS
Epitope
pThr2609
(36), (15), (14), (14), (14), (11), (8), (7), (7), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(273), (121), (56), (40), (29), (13), (12), (4), (3), (1), (1), (1)
Host
Rabbit
(219), (58), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(15), (12), (12), (8), (8), (8), (8), (8), (8), (8), (8), (4), (4), (4)
Application
Western Blotting (WB)
(125), (82), (80), (74), (60), (46), (45), (17), (14), (9), (2), (2), (1), (1)
Pubmed 3 references available
Quantity 100 μL
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Catalog No. ABIN362178
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Immunogen Peptide sequence around phosphorylation site of pThr2609 (V-E-T (p) -Q-A) derived from Human DNA-PK. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of DNA PKcs only when phosphorylated at threonine 2609.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name DNA PKcs
Background The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity, PRKDC must be bound to DNA to express its catalytic properties
Molecular Weight 450 kDa
NCBI Accession NP_001075
UniProt P78527
Research Area Chromatin and Nuclear Signaling, DNA/RNA, Signaling, Apoptosis/Necrosis
Application Notes Western blotting: 1:500-1:1000
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Douglas, Sapkota, Morrice et al.: "Identification of in vitro and in vivo phosphorylation sites in the catalytic subunit of the DNA-dependent protein kinase." in: The Biochemical journal, Vol. 368, Issue Pt 1, pp. 243-51, 2002 (PubMed).

Chan, Chen, Prithivirajsingh et al.: "Autophosphorylation of the DNA-dependent protein kinase catalytic subunit is required for rejoining of DNA double-strand breaks." in: Genes & development, Vol. 16, Issue 18, pp. 2333-8, 2002 (PubMed).

Ding, Reddy, Wang et al.: "Autophosphorylation of the catalytic subunit of the DNA-dependent protein kinase is required for efficient end processing during DNA double-strand break repair." in: Molecular and cellular biology, Vol. 23, Issue 16, pp. 5836-48, 2003 (PubMed).

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