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Antibody
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anti-Protein Kinase AMP-Activated alpha 1 Catalytic Subunit PRKAA1 pSer487
Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (pSer487) antibody
| Antigen |
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| Synonyms |
PRKAA1, AMPK, AMPK1 |
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Epitope
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
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| Catalog no. |
ABIN362185 |
| Quantity |
50µg (50µg/50µl) (Variants) |
| Price |
280.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business days |
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Alternative name
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AMPKalpha1 (Phospho-Ser487)
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Swiss-Prot
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Q13131 P54646
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Immunogen
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The antiserum was produced against synthesized phosphopeptide derived from humanAMPKalpha1around the phosphorylation site of serine 487 (S-G- S P -V-S).
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Isotype
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IgG (Matching secondary antibodies)
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Specificity
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AMPKalpha1 (Phospho-Ser487) (Human) antibody detects endogenous levels of AMPKalpha1 only whenphosphorylated at serine 487.
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Application Notes
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WB: 1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200
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Concentration
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50µg/50µl
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Purification
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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Buffer
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Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage
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Store at -20°C. Avoid repeat freeze-thaw cycles.
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Restrictions
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For Research Use only
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Alternatives for antigen "Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1)", type "Antibodies"
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Hosts
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Rabbit (26), Mouse (1)
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Reactivities
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Human (27), Rat (Rattus) (19), Mouse (Murine) (18), Cow (Bovine) (1)
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Applications
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Western Blotting (WB) (23), Immunohistochemistry (IHC) (18), ELISA (8), Immunofluorescence (IF) (4), Immunoprecipitation (IP) (2), Enzyme Immunoassay (EIA) (1), Flow Cytometry (FACS) (1), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (1)
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Epitopes
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pSer485/Ser491 (3), N-Term (2), pThr174 (2), pThr174/Thr172 (2), C-Term (1), pSer487 (1), pT172 (1)
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