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ASK1 antibody (pSer966)
MAP3K5
Reactivity: Human, Mouse
WB, IHC
Host: Rabbit
Polyclonal
unconjugated
Product Details anti-ASK1 Antibody
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Target
See all ASK1 (MAP3K5) Antibodies
ASK1 (MAP3K5)
(Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))
Binding Specificity
All epitopes for ASK1 antibodies
pSer966
Reactivity
All reactivities for ASK1 antibodies
Human, Mouse
Host
All hosts for ASK1 antibodies
Rabbit
Clonality
All clonalities for ASK1 antibodies
Polyclonal
Conjugate
All conjugates for ASK1 antibodies
This ASK1 antibody is un-conjugated
Application
All applications for ASK1 antibodies
Western Blotting (WB), Immunohistochemistry (IHC)
Specificity
The antibody detects endogenous level of ASK1 only when phosphorylated at serine 966.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Immunogen
Peptide sequence around phosphorylation site of pSer966 (S-I-S (p) -L-P) derived from Human ASK1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype
IgG
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Alternatives
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Application Details
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Application Notes
Western blotting: 1:500-1:1000 Immunohistochemistry: 1:50-1:100
Restrictions
For Research Use only
Handling
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Format
Liquid
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
References for anti-ASK1 antibody (ABIN362196)
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Yang, Yu, Guan, Gu, Cao, Wang, Zheng, Xu, Shen, Liu : "ASK1-JNK signaling cascade mediates Ad-ST13-induced apoptosis in colorectal HCT116 cells. " in: Journal of cellular biochemistry , Vol. 110 , Issue 3 , pp. 581-8 , (2010 ) (PubMed ).
Target Details for ASK1
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Target
ASK1 (MAP3K5)
(Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))
Alternative Name
ASK1 (MAP3K5 Products )
Synonyms
ASK1 antibody, MAPKKK5 antibody, MEKK5 antibody, mitogen-activated protein kinase kinase kinase 5 antibody, MUD21.11 antibody, MUD21_11 antibody, 7420452D20Rik antibody, ASK antibody, Mekk5 antibody, Ask1 antibody, RGD1306565 antibody, mitogen-activated protein kinase kinase kinase 5 antibody, MAP3K5 antibody, MAPKKK5 antibody, Map3k5 antibody, map3k5 antibody
Background
Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively.
Molecular Weight
155 kDa
NCBI Accession
NP_005914
UniProt
Q99683
Pathways
MAPK Signaling , Positive Regulation of Endopeptidase Activity , Unfolded Protein Response
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