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Histone Deacetylase 4 (HDAC4) (pSer632) antibody

Details for Product No. ABIN362215, Supplier: Login to see
Antigen
Epitope
pSer632
47
34
20
15
10
10
6
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
260
102
71
8
3
3
2
2
1
1
1
1
1
Host
Rabbit
227
39
2
Clonality
Polyclonal
Conjugate
Un-conjugated
10
9
9
4
4
4
3
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
236
85
50
32
30
22
13
12
11
5
3
1
1
1
1
Supplier
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Immunogen Peptide sequence around phosphorylation site of pSer632 (A-Q-S (p) -S-P) derived from Human HDAC4. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of HDAC4 only when phosphorylated at serine 632.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name HDAC4 (HDAC4 Antibody Abstract)
Background Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.
Molecular Weight 140 kDa
NCBI Accession NP_006028
UniProt P56524
Research Area Cardiovascular, Hypertrophy, Wnt, DNA/RNA, Transcription Factors
Pathways
Application Notes Western blotting: 1:500-1:1000  
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Product cited in: Lim, Luo, Koh et al.: "Distinct mechanisms involving diverse histone deacetylases repress expression of the two gonadotropin beta-subunit genes in immature gonadotropes, and their actions are overcome by ..." in: Molecular and cellular biology, Vol. 27, Issue 11, pp. 4105-20, 2007 (PubMed).

Background publications Wang, Kruhlak, Wu et al.: "Regulation of histone deacetylase 4 by binding of 14-3-3 proteins." in: Molecular and cellular biology, Vol. 20, Issue 18, pp. 6904-12, 2000 (PubMed).

Grozinger, Schreiber: "Regulation of histone deacetylase 4 and 5 and transcriptional activity by 14-3-3-dependent cellular localization." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 14, pp. 7835-40, 2000 (PubMed).