CDK1 antibody (pTyr15)

Catalog No. ABIN362298

There are 2+ publications for this product available. The Rabbit Polyclonal anti-CDK1 antibody is suitable to detect CDK1 in samples from Human, Mouse and Rat.

Quick Overview for CDK1 antibody (pTyr15) (ABIN362298)

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK1 antibody is un-conjugated

Application: Please inquire

Product Details anti-CDK1 Antibody

Binding Specificity: pTyr15

Specificity: The antibody detects endogenous level of CDC2 only when phosphorylated at tyrosine 15.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pTyr15 (G-T-Y (p) -G-V) derived from Human CDC2. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-CDK1 antibody (ABIN362298)

Kubara, Kernéis-Golsteyn, Studény, Lanser, Meijer, Golsteyn: "Human cells enter mitosis with damaged DNA after treatment with pharmacological concentrations of genotoxic agents." in: The Biochemical journal, Vol. 446, Issue 3, pp. 373-81, (2012) (PubMed).

Yao, Li, Liu, Huang, Guo: "SUMOylation-regulated protein phosphorylation, evidence from quantitative phosphoproteomics analyses." in: The Journal of biological chemistry, Vol. 286, Issue 31, pp. 27342-9, (2011) (PubMed).

Target Details for CDK1

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Alternative Name: CDC2

Background: Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.

Molecular Weight: 34 kDa

NCBI Accession: NP_001163

UniProt: P06493

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA