MAPK14 antibody (pTyr182)

Catalog No. ABIN362313

Cited in 3+ publications. The Rabbit Polyclonal anti-MAPK14 antibody (ABIN362313) specifically detects MAPK14 in WB and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for MAPK14 antibody (pTyr182) (ABIN362313)

Target: MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAPK14 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-MAPK14 Antibody

Binding Specificity: pTyr182

Specificity: The antibody detects endogenous level of P38MAPK only when phosphorylated at tyrosine 182.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pTyr182 (T-G-Y (p) -V-A) derived from Human P38 MAPK. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-MAPK14 antibody (ABIN362313)

Su, Gu, Wang, Wang: "Lidocaine attenuates proinflammatory cytokine production induced by extracellular adenosine triphosphate in cultured rat microglia." in: Anesthesia and analgesia, Vol. 111, Issue 3, pp. 768-74, (2010) (PubMed).

Mashima, Sato, Sugimoto, Tsuruo, Seimiya: "Promotion of glioma cell survival by acyl-CoA synthetase 5 under extracellular acidosis conditions." in: Oncogene, Vol. 28, Issue 1, pp. 9-19, (2009) (PubMed).

Chen, Song, Zhou, Tong, Li, Fu, Guo, Dong, He, Qiao, Zhan, Li: "Disruption of xCT inhibits cancer cell metastasis via the caveolin-1/beta-catenin pathway." in: Oncogene, Vol. 28, Issue 4, pp. 599-609, (2009) (PubMed).

Target Details for MAPK14

Target: MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))

Alternative Name: P38 MAPK

Background: Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.

Molecular Weight: 43 kDa

UniProt: Q16539

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Hepatitis C, Toll-Like Receptors Cascades, Autophagy, Thromboxane A2 Receptor Signaling, BCR Signaling, S100 Proteins