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Antibodies
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anti-Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) (pSer424) antibody
Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) (pSer424) antibody
| Antigen |
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| Synonyms |
S6K, PS6K, S6K1, STK14A, p70-S6K, p70-alpha, p70(S6K)-alpha |
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Epitope
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pSer411 (10), pSer424 (10), pThr421 (9), pThr421/pSer424 (4), C-Term (3), Center (2), N-Term (2), pSer418 (2), pSer434 (2), pThr389 (2), S404 (1), S411 (1), S418 (1), S424 (1), T421 (1), pS418 (1), pS424 (1), pSer447 (1), pThr444 (1)
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Human (187), Mouse (Murine) (116), Rat (Rattus) (107), Cow (Bovine) (25), Dog (Canine) (20), Pig (Porcine) (18), Chicken (16), Monkey (2), Rabbit (2), Sheep (Ovine) (2), Cat (Feline) (1), Horse (Equine) (1), Xenopus laevis (1)
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Application
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 Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Western Blotting (WB) (136), Immunofluorescence (IF) (83), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (55), Immunohistochemistry (IHC) (53), Flow Cytometry (FACS) (36), Enzyme Immunoassay (EIA) (34), ELISA (28), Immunoprecipitation (IP) (12), Immunohistochemistry (Frozen Sections) (IHC (fro)) (6), Dot Blot (Dot) (4), ELISA (Detection) (2), Immunoelectron Microscopy (IEM) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2)
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| Catalog no. |
ABIN362363 |
| Quantity |
50µg (50µg/50µl) (Variants) |
| Price |
280.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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p70 S6 Kinase (Phospho-Ser424)
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Swiss-Prot
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P23443
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Immunogen
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The antiserum was produced against synthesized phosphopeptide derived from humanp70 S6 Kinase around the phosphorylation site of serine 424 (P-V-S P -P-V).
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Isotype
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IgG (Matching secondary antibodies)
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Specificity
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P70 S6 Kinase (Phospho-Ser424) (Human) antibody detects endogenous levels of p70 S6 Kinaseonly when phosphorylated at serine 424.
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Application Notes
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WB: 1:500~1:1000 IHC: 1:50-1:100 IF:1:100~1:200
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Concentration
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50µg/50µl
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Purification
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
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Buffer
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Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage
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Store at -20°C. Avoid repeat freeze-thaw cycles.
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Restrictions
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For Research Use only
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Alternatives for antigen "Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1)", type "Antibodies"
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Hosts
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Rabbit (171), Mouse (15), Goat (6)
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Reactivities
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Human (187), Mouse (Murine) (116), Rat (Rattus) (107), Cow (Bovine) (25), Dog (Canine) (20), Pig (Porcine) (18), Chicken (16), Monkey (2), Rabbit (2), Sheep (Ovine) (2), Cat (Feline) (1), Horse (Equine) (1), Xenopus laevis (1)
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Applications
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Western Blotting (WB) (136), Immunofluorescence (IF) (83), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (55), Immunohistochemistry (IHC) (53), Flow Cytometry (FACS) (36), Enzyme Immunoassay (EIA) (34), ELISA (28), Immunoprecipitation (IP) (12), Immunohistochemistry (Frozen Sections) (IHC (fro)) (6), Dot Blot (Dot) (4), ELISA (Detection) (2), Immunoelectron Microscopy (IEM) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2)
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Conjugates
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Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2), Alexa Flour 488 (1)
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Epitopes
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pSer411 (10), pSer424 (10), pThr421 (9), pThr421/pSer424 (4), C-Term (3), Center (2), N-Term (2), pSer418 (2), pSer434 (2), pThr389 (2), S404 (1), S411 (1), S418 (1), S424 (1), T421 (1), pS418 (1), pS424 (1), pSer447 (1), pThr444 (1)
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