CAMKII gamma antibody (pThr286)

Catalog No. ABIN362370

The Rabbit Polyclonal anti-CAMKII gamma antibody is suitable to detect CAMKII gamma in samples from Human, Rat and Mouse. It has been validated for WB and IF.

Quick Overview for CAMKII gamma antibody (pThr286) (ABIN362370)

Target: CAMKII gamma (CAMK2G) (Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CAMKII gamma antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-CAMKII gamma Antibody

Binding Specificity: pThr286

Specificity: The antibody detects endogenous level of CaMKII only when phosphorylated at threonine 286.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pThr286 (Q-E-T (p) -V-D) derived from Human CaMKII. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for CAMKII gamma

Target: CAMKII gamma (CAMK2G) (Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G))

Alternative Name: CaMKII

Background: CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity

Molecular Weight: 50 kDa

UniProt: P11798

Pathways: WNT Signaling, Interferon-gamma Pathway, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of long-term Neuronal Synaptic Plasticity