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Antibodies
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anti-Cytokeratin 18 (KRT18) (pSer33) antibody
Cytokeratin 18 (KRT18) (pSer33) antibody
| Antigen |
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| Synonyms |
K18, CK18, Krt1-18, CYK18, KRT18, LOC100008885, MGC109143, krt18 |
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Epitope
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Human (141), Rat (Rattus) (57), Mouse (Murine) (56), Dog (Canine) (41), Rabbit (35), Cow (Bovine) (23), Pig (Porcine) (22), Chicken (20), Horse (Equine) (17), Mammalian (10), Hamster (5), None (4), Sheep (Ovine) (3), Bird (Avian) (2), Cat (Feline) (2), Monkey (1)
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Application
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 Alternatives Western Blotting (WB), Immunohistochemistry (IHC)
Western Blotting (WB) (83), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (77), Flow Cytometry (FACS) (67), Immunofluorescence (IF) (67), Immunohistochemistry (IHC) (32), Immunohistochemistry (Frozen Sections) (IHC (fro)) (28), Enzyme Immunoassay (EIA) (22), ELISA (20), Immunoprecipitation (IP) (18), Immunocytochemistry (ICC) (12), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), ELISA (Detection) (2), Immunoelectron Microscopy (IEM) (2)
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| Catalog no. |
ABIN362391 |
| Quantity |
50µg (50µg/50µl) (Variants) |
| Price |
280.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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Keratin 18 (Phospho-Ser33)
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Swiss-Prot
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P05783
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Immunogen
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The antiserum was produced against synthesized phosphopeptide derived from human Keratin 18 around the phosphorylation site of serine 33 (A-A-S P -V-Y).
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Isotype
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IgG (Matching secondary antibodies)
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Specificity
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Keratin 18 (Phospho-Ser33) (Human) antibody detects endogenous levels of Keratin 18 only when phosphorylated at serine 33.
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Application Notes
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WB: 1:500~1:1000 IHC: 1:50~1:100
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Concentration
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50µg/50µl
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Purification
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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Buffer
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Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage
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Store at -20°C. Avoid repeat freeze-thaw cycles.
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Restrictions
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For Research Use only
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Alternatives for antigen "Cytokeratin 18 (KRT18)", type "Antibodies"
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Hosts
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Mouse (89), Rabbit (77), Guinea Pig (1), Sheep (1)
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Reactivities
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Human (141), Rat (Rattus) (57), Mouse (Murine) (56), Dog (Canine) (41), Rabbit (35), Cow (Bovine) (23), Pig (Porcine) (22), Chicken (20), Horse (Equine) (17), Mammalian (10), Hamster (5), None (4), Sheep (Ovine) (3), Bird (Avian) (2), Cat (Feline) (2), Monkey (1)
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Applications
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Western Blotting (WB) (83), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (77), Flow Cytometry (FACS) (67), Immunofluorescence (IF) (67), Immunohistochemistry (IHC) (32), Immunohistochemistry (Frozen Sections) (IHC (fro)) (28), Enzyme Immunoassay (EIA) (22), ELISA (20), Immunoprecipitation (IP) (18), Immunocytochemistry (ICC) (12), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), ELISA (Detection) (2), Immunoelectron Microscopy (IEM) (2)
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Conjugates
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FITC (13), Biotin (11), Alexa Fluor 488 (3), HRP (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2), Alexa Fluor 700 (1)
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Epitopes
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pSer33 (6), C-Term (5), Center (1), N-Term (1)
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