»
Antibodies
»
anti-Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2) (pThr3...
Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2) (pThr334) antibody
| Antigen |
|
| Synonyms |
MK2, Rps6kc1, AA960234, mapkapk2, wu:fb30e10, wu:fc56c10, wu:fi03a01, MGC78852, MAPKAPK2 |
|
Epitope
|
|
| Clonality |
Polyclonal |
|
Host
|
|
|
Reactivity
|
Human (74), Rat (Rattus) (50), Mouse (Murine) (47), Cow (Bovine) (20), Rabbit (17), Pig (Porcine) (15), Chicken (3), Dog (Canine) (3), Hamster (2), Horse (Equine) (1), Xenopus laevis (1)
|
|
Application
|
 Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Western Blotting (WB) (56), Immunofluorescence (IF) (29), Flow Cytometry (FACS) (18), Enzyme Immunoassay (EIA) (17), Immunohistochemistry (IHC) (16), ELISA (13), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13), Immunoprecipitation (IP) (8), Dot Blot (Dot) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
|
| Catalog no. |
ABIN362395 |
| Quantity |
50µg (50µg/50µl) (Variants) |
| Price |
280.00 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 5 Business Days |
|
Alternative name
|
MAPKAPK-2 (Phospho-Thr334)
|
|
Swiss-Prot
|
P49137
|
|
Immunogen
|
The antiserum was produced against synthesized phosphopeptide derived from humanMAPKAPK-2 around the phosphorylation site of threonine 334 (P-Q-T P -P-L).
|
|
Isotype
|
IgG (Matching secondary antibodies)
|
|
Specificity
|
MAPKAPK-2 (Phospho-Thr334) (Human) antibody detects endogenous levels of MAPKAPK-2 onlywhen phosphorylated at threonine 334.
|
|
Application Notes
|
WB: 1:500~1:1000 IHC: 1:50-1:100 IF:1:100~1:200
|
|
Concentration
|
50µg/50µl
|
|
Purification
|
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
|
|
Buffer
|
Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
|
|
Storage
|
Store at -20°C. Avoid repeat freeze-thaw cycles.
|
|
Restrictions
|
For Research Use only
|
Alternatives for antigen "Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2)", type "Antibodies"
|
Hosts
|
Rabbit (69), Mouse (7)
|
|
Reactivities
|
Human (74), Rat (Rattus) (50), Mouse (Murine) (47), Cow (Bovine) (20), Rabbit (17), Pig (Porcine) (15), Chicken (3), Dog (Canine) (3), Hamster (2), Horse (Equine) (1), Xenopus laevis (1)
|
|
Applications
|
Western Blotting (WB) (56), Immunofluorescence (IF) (29), Flow Cytometry (FACS) (18), Enzyme Immunoassay (EIA) (17), Immunohistochemistry (IHC) (16), ELISA (13), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13), Immunoprecipitation (IP) (8), Dot Blot (Dot) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
|
|
Conjugates
|
Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
|
|
Epitopes
|
pThr334 (7), Ab-334 (5), pSer272 (4), C-Term (2), N-Term (2), pS272 (1), pThr222 (1)
|
