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SMAD, Mothers Against DPP Homolog 3 (SMAD3) (pSer425) antibody

Antigen

SMAD, Mothers Against DPP Homolog 3 (SMAD3)
Synonyms MADH3, JV15-2, HSPC193, HsT17436, MGC60396, DKFZp586N0721, DKFZp686J10186
Epitope
Alternatives

pSer425
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Application
Alternatives Immunohistochemistry (IHC)
Catalog no. ABIN362419
Quantity 50µg  (50µg/50µl)  (Variants)
Price 280.00 $   Plus shipping costs $35.00
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Additional Information
Application Details Alternatives Secondary Antibodies Variants back to top

Alternative name Smad3 (Phospho-Ser425)
Swiss-Prot P84022
Immunogen The antiserum was produced against synthesized phosphopeptide derived from humanSmad3 around the phosphorylation site of serine 425 (C-S-S-V-S P ).
Isotype IgG  (Matching secondary antibodies)
Specificity Smad3 (Phospho-Ser425) (Human) antibody detects endogenous levels of Smad3 only whenphosphorylated at serine 425.

Application Details
Alternatives Secondary Antibodies Variants back to top

Application Notes IHC: 1:50~1:100
Concentration 50µg/50µl
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Buffer Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C. Avoid repeat freeze-thaw cycles.
Restrictions For Research Use only

Alternatives
Application Details Secondary Antibodies Variants back to top

Alternatives for antigen "SMAD, Mothers Against DPP Homolog 3 (SMAD3)", type "Antibodies"
Hosts Rabbit (84), Mouse (20)
Reactivities Human (96), Rat (Rattus) (56), Mouse (Murine) (51), Cow (Bovine) (19), Chicken (15)
Applications Western Blotting (WB) (56), Immunofluorescence (IF) (36), Immunohistochemistry (IHC) (30), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (29), Enzyme Immunoassay (EIA) (27), ELISA (20), Flow Cytometry (FACS) (18), Immunoprecipitation (IP) (10), Dot Blot (Dot) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
Epitopes pSer425 (5), Center D201 (1), Center N206 (1), S204 (1), S208 (1), S213 (1), pS423 pS425 (1), pSer204 (1), pSer208 (1), pSer213 (1), pSer423, pSer425 (1)