TRKB antibody (pTyr705)

Catalog No. ABIN362425

This Rabbit Polyclonal antibody specifically detects TRKB in WB. It exhibits reactivity toward Human, Rat and Mouse. It has been mentioned in 2+ publications

Quick Overview for TRKB antibody (pTyr705) (ABIN362425)

Target: TRKB (NTRK2) (Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRKB antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TRKB Antibody

Binding Specificity: pTyr705

Specificity: The antibody detects endogenous level of TrkB only when phosphorylated at tyrosine 705.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pTyr705 (T-D-Y P-Y-R) derived from Human TrkB. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-TRKB antibody (ABIN362425)

Hill, Wu, Gogos, van den Buuse: "Sex-dependent alterations in BDNF-TrkB signaling in the hippocampus of reelin heterozygous mice: a role for sex steroid hormones." in: Journal of neurochemistry, Vol. 126, Issue 3, pp. 389-99, (2013) (PubMed).

Hill, van den Buuse: "Sex-dependent and region-specific changes in TrkB signaling in BDNF heterozygous mice." in: Brain research, Vol. 1384, pp. 51-60, (2011) (PubMed).

Target Details for TRKB

Target: TRKB (NTRK2) (Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2))

Alternative Name: TrkB

Background: Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.

Molecular Weight: 140 kDa

UniProt: Q16620

Pathways: RTK Signaling, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Skeletal Muscle Fiber Development, Feeding Behaviour, Dicarboxylic Acid Transport