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Cofilin 1 (Non-Muscle) (CFL1) (pTyr139) antibody

Details for Product No. ABIN362439, Supplier: Login to see
Antigen
Epitope
pTyr139
51
21
20
14
14
13
12
11
8
6
5
4
4
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
256
132
119
7
7
4
4
2
2
1
1
Host
Rabbit
219
34
3
Clonality
Polyclonal
Conjugate
Un-conjugated
8
7
7
5
4
4
4
3
3
3
3
3
3
3
3
Application
Immunohistochemistry (IHC)
169
93
77
46
37
37
18
14
11
4
3
2
2
1
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Immunogen Peptide sequence around phosphorylation site of pTyr139 (N-C-Y (p) -E-E) derived from Human cofilin. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of cofilin1 only when phosphorylated at tyrosine 139.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name cofilin1 (CFL1 Antibody Abstract)
Background Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
Molecular Weight 19 kDa
NCBI Accession NP_005498
UniProt P23528
Pathways
Application Notes Immunohistochemistry: 1:50-1:100
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Chen, Cizeau, Vande Velde et al.: "Nix and Nip3 form a subfamily of pro-apoptotic mitochondrial proteins." in: The Journal of biological chemistry, Vol. 274, Issue 1, pp. 7-10, 1999 (PubMed).

Yang, Higuchi, Ohashi et al.: "Cofilin phosphorylation by LIM-kinase 1 and its role in Rac-mediated actin reorganization." in: Nature, Vol. 393, Issue 6687, pp. 809-12, 1998 (PubMed).

Arber, Barbayannis, Hanser et al.: "Regulation of actin dynamics through phosphorylation of cofilin by LIM-kinase." in: Nature, Vol. 393, Issue 6687, pp. 805-9, 1998 (PubMed).