Spleen tyrosine Kinase (SYK) (pTyr323) antibody

Details for Product No. ABIN362477
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Antigen
Synonyms p72-Syk, Sykb, p72syk
Epitope
pTyr323
(38), (27), (19), (12), (10), (7), (7), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(231), (102), (96), (37), (27), (25), (12), (12)
Host
Rabbit
(174), (57), (7)
Clonality
Polyclonal
Conjugate
Un-conjugated
(6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application
Immunohistochemistry (IHC), Immunofluorescence (IF)
(181), (83), (76), (72), (50), (33), (30), (24), (3), (3), (2), (1), (1), (1)
Pubmed 4 references available
Catalog no. ABIN362477
Quantity 50 µL
Price
198.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Peptide sequence around phosphorylation site of pTyr323 (N-P-Y (p) -E- P) derived from Human syk. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of syk only when phosphorylated at tyrosine 323.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name syk
Background Positive effector of BCR-stimulated responses. Couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-348 and Tyr-352. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion
Molecular Weight 72 kDa
NCBI Accession NP_001128
UniProt P43405
Application Notes Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Law, Chandran, Sidorenko et al.: "Phospholipase C-gamma1 interacts with conserved phosphotyrosyl residues in the linker region of Syk and is a substrate for Syk." in: Molecular and cellular biology, Vol. 16, Issue 4, pp. 1305-15, 1996 (PubMed).

Deckert, Elly, Altman et al.: "Coordinated regulation of the tyrosine phosphorylation of Cbl by Fyn and Syk tyrosine kinases." in: The Journal of biological chemistry, Vol. 273, Issue 15, pp. 8867-74, 1998 (PubMed).

Turner, Schweighoffer, Colucci et al.: "Tyrosine kinase SYK: essential functions for immunoreceptor signalling." in: Immunology today, Vol. 21, Issue 3, pp. 148-54, 2000 (PubMed).

Zhang, Billingsley, Kincaid et al.: "Phosphorylation of Syk activation loop tyrosines is essential for Syk function. An in vivo study using a specific anti-Syk activation loop phosphotyrosine antibody." in: The Journal of biological chemistry, Vol. 275, Issue 45, pp. 35442-7, 2000 (PubMed).

Hosts (174), (57), (7)
Reactivities (231), (102), (96), (37), (27), (25), (12), (12)
Applications (181), (83), (76), (72), (50), (33), (30), (24), (3), (3), (2), (1), (1), (1)
Conjugates (6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Epitopes (38), (27), (19), (12), (10), (7), (7), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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