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ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa) (AA 491-495)

Details for Product anti-ZAP70 Antibody No. ABIN362797, Supplier: Login to see New
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Antigen
Alternatives
Epitope
AA 491-495
(54), (51), (51), (30), (22), (22), (22), (21), (15), (15), (15), (14), (13), (5), (5), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(502), (178), (122), (4), (4), (3), (2), (2), (2), (1)
Host
Rabbit
(325), (158), (15), (8), (2)
Clonality
Polyclonal
Conjugate
This ZAP70 antibody is un-conjugated
(32), (24), (18), (14), (10), (8), (5), (5), (4), (4), (4), (4), (4), (4), (4), (1), (1)
Application
Immunohistochemistry (IHC)
(307), (166), (157), (95), (79), (47), (46), (44), (15), (7), (6), (5), (4), (3), (2)
Pubmed 3 references available
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Quantity 50 μL
Shipping to United States ( )
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Immunogen Peptide sequence around AA 491-495 (S-Y-Y-T-A) derived from Human ZAP70. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of total Zap-70 protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative Name Zap-70 (ZAP70 Antibody Abstract)
Background ZAP70 is a 70-kD tyrosine phosphoprotein that associates with the zeta chain and undergoes tyrosine phosphorylation following TCR stimulation. The ZAP70 gene is expressed in T- and natural KILLER cells. Protein-Tyrosine Kinases (PTKs) play an integral role in T-cell activation. Stimulation of the T-cell antigen receptor results in tyrosine phosphorylation of a number of cellular substrates. One of these is the TCR-zeta chain, which can mediate the transduction of extracellular stimuli into cellular effector functions
Molecular Weight 70 kDa
UniProt P43403
Research Area Signaling, Tags/Labels, Immunology, Cancer
Pathways TCR Signaling
Application Notes Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Salomon, Ficarro, Brill et al.: "Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 2, pp. 443-8, 2003 (PubMed).

Kong, Dalton, Bubeck Wardenburg et al.: "Distinct tyrosine phosphorylation sites in ZAP-70 mediate activation and negative regulation of antigen receptor function." in: Molecular and cellular biology, Vol. 16, Issue 9, pp. 5026-35, 1996 (PubMed).

Chan, Dalton, Johnson et al.: "Activation of ZAP-70 kinase activity by phosphorylation of tyrosine 493 is required for lymphocyte antigen receptor function." in: The EMBO journal, Vol. 14, Issue 11, pp. 2499-508, 1995 (PubMed).

Catalog No. ABIN362797
$187.00
Plus shipping costs $45.00

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