PRKAA1/PRKAA2 antibody (AA 170-174, AA 172-176)
Quick Overview for PRKAA1/PRKAA2 antibody (AA 170-174, AA 172-176) (ABIN362825)
Target
Reactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- AA 170-174, AA 172-176
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Specificity
- The antibody detects endogenous level of total AMPKa1/AMPKa2 protein.
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Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.
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Immunogen
- Peptide sequence around AA 172-176, 170-174 (L-R-T-S-C) derived from Human AMPKa1, AMPKa2. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
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Isotype
- IgG
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anti-AMPK1/AMPK2 (pThr172), (pThr183) antibody
Reactivity: Human, Mouse, Rat, Monkey WB, IF, ELISA, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-AMPK1/AMPK2 (pThr172), (pThr183) antibodyReactivity: Human, Mouse, Rat, Pig, Sheep WB, ELISA, IF (cc), IHC (p), FACS, IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-AMPK1/AMPK2 (AA 14-47), (N-Term) antibodyReactivity: Mouse WB Host: Rabbit Polyclonal RB43852 unconjugated
anti-AMPK1/AMPK2 (Ser1071) antibodyReactivity: Human, Mouse, Rat, Monkey WB, IHC, ELISA Host: Rabbit Polyclonal unconjugated
anti-AMPK1/AMPK2 (pThr172), (pThr183) antibody (Cy7)Reactivity: Human, Mouse, Rat, Pig, Sheep WB, IF (cc), FACS, IF (p) Host: Rabbit Polyclonal Cy7
anti-AMPK1/AMPK2 (Catalytic Domain), (N-Term) antibodyReactivity: Human, Mouse, Rat, Drosophila melanogaster, Vertebrate WB, IF, ELISA, IHC (fro), IP Host: Mouse Monoclonal 84-1 unconjugated
anti-AMPK1/AMPK2 (pThr172), (pThr183) antibodyReactivity: Human, Mouse, Rat, Pig, Monkey, Penaeus japonicus WB, IHC, IF, ELISA Host: Rabbit Polyclonal unconjugated
anti-AMPK1/AMPK2 (Center) antibodyReactivity: Human, Mouse, Rat, Pig, Chicken WB, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-AMPK1/AMPK2 (pThr172), (pThr183) antibodyReactivity: Human, Mouse, Rat, Pig, Chicken WB, IHC Host: Rabbit Polyclonal unconjugated
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Application Notes
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Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200 -
Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C/-20 °C
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Storage Comment
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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- PRKAA1/PRKAA2 (AMPK1/AMPK2)
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Alternative Name
- AMPKalpha1/AMPKalpha2
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Background
- Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
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Molecular Weight
- 63 kDa
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NCBI Accession
- NP_006243, NP_006242
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UniProt
- Q13131
Target
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