PRKAA1/PRKAA2 antibody (pThr172, pThr183)

Catalog No. ABIN7201115

This Rabbit Polyclonal antibody specifically detects PRKAA1/PRKAA2 in WB, IHC, IF and ELISA. It exhibits reactivity toward Human, Mouse, Rat, Pig, Monkey and Penaeus japonicus.

Quick Overview for PRKAA1/PRKAA2 antibody (pThr172, pThr183) (ABIN7201115)

Target: PRKAA1/PRKAA2 (AMPK1/AMPK2)

Reactivity: Human, Mouse, Rat, Pig, Monkey, Penaeus japonicus

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKAA1/PRKAA2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Product Details anti-PRKAA1/PRKAA2 Antibody

Binding Specificity: pThr172, pThr183

Purpose: Rabbit Anti-AMPKα1/2 (phospho Thr183/172) Polyclonal Antibody

Specificity: Phospho-AMPKα1/2 (T183/172) Polyclonal Antibody detects endogenous levels of AMPKα1/2 protein only when phosphorylated at T183/172.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human AMPKalpha1/2 Phospho-Thr183/172

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IF 1:50-200,WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:40000,Not yet tested in other applications,

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PRKAA1/PRKAA2

Target: PRKAA1/PRKAA2 (AMPK1/AMPK2)

Alternative Name: AMPKalpha1/2

Background: PRKAA1, AMPK1, 5'-AMP-activated protein kinase catalytic subunit alpha-1, AMPK subunit alpha-1, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, Tau-protein kinase PRKAA1, PRKAA2, AMPK,Protein kinase AMP-activated catalytic subunit alpha 1 encoded by PRKAA1 belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Molecular Weight: 63kD

Gene ID: 5562, 5563

UniProt: Q13131, P54646