MSK1 antibody (AA 374-378)

Catalog No. ABIN362837

The Rabbit Polyclonal anti-MSK1 antibody is suitable to detect MSK1 in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for MSK1 antibody (AA 374-378) (ABIN362837)

Target: MSK1 (RPS6KA5) (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 5 (RPS6KA5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MSK1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MSK1 Antibody

Binding Specificity: AA 374-378

Specificity: The antibody detects endogenous level of total MSK1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Peptide sequence around AA 374-378 (G-Y-S-F-V) derived from Human MSK1. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for MSK1

Target: MSK1 (RPS6KA5) (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 5 (RPS6KA5))

Alternative Name: MSK1

Background: Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).

Molecular Weight: 90 kDa

NCBI Accession: NP_004746

UniProt: O75582

Pathways: MAPK Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades