The Rabbit Polyclonal anti-MEK1 + MEK2 antibody (ABIN362847) specifically detects MEK1 + MEK2 in WB, IHC and IF.
The antibody is reactive with Human, Mouse and Rat samples.
Quick Overview for MEK1 + MEK2 antibody (AA 215-219, AA 219-223) (ABIN362847)
Target
MEK1 + MEK2
Reactivity
Human, Mouse, Rat
Host
Rabbit
Clonality
Polyclonal
Conjugate
This MEK1 + MEK2 antibody is un-conjugated
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Binding Specificity
AA 215-219, AA 219-223
Specificity
The antibody detects endogenous level of total MEK1/MEK2 protein.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.
Immunogen
Peptide sequence around AA 215-219, 219-223 (I-D-S-M-A) derived from Human MEK1, MEK2. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Western blotting: 1:500-1:1000 Immunohistochemistry: 1:50-1:100 Immunofluorescence: 1:100-1:200
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Target
MEK1 + MEK2
Alternative Name
MEK1/MEK2
Background
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.