MEK1 antibody (AA 289-293)

Catalog No. ABIN362997

Cited in 1 publication. The Rabbit Polyclonal anti-MEK1 antibody (ABIN362997) specifically detects MEK1 in WB and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for MEK1 antibody (AA 289-293) (ABIN362997)

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MEK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-MEK1 Antibody

Binding Specificity: AA 289-293

Specificity: The antibody detects endogenous level of total MEK1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Peptide sequence around AA 289-293 (P-R-T-P-G) derived from Human MEK1. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-MEK1 antibody (ABIN362997)

Xu, Zhao, Huang, Ding, Wang, Wang, Cheng, Kang, Wang: "CD40-expressing plasmid induces anti-CD40 antibody and enhances immune responses to DNA vaccination." in: The journal of gene medicine, Vol. 12, Issue 1, pp. 97-106, (2010) (PubMed).

Target Details for MEK1

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Alternative Name: MEK1

Background: Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.

Molecular Weight: 45 kDa

NCBI Accession: NP_002746

UniProt: Q02750

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling