NFKBIB antibody (pSer23)

Catalog No. ABIN363133

This Rabbit Polyclonal antibody specifically detects NFKBIB in IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for NFKBIB antibody (pSer23) (ABIN363133)

Target: NFKBIB (NF-kappa-B inhibitor beta (NFKBIB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFKBIB antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-NFKBIB Antibody

Binding Specificity: pSer23

Specificity: The antibody detects endogenous level of IkB-b only when phosphorylated at serine 23.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pSer23 (L-G-S (p) -L-G) derived from Human IkB-b. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry: 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for NFKBIB

Target: NFKBIB (NF-kappa-B inhibitor beta (NFKBIB))

Alternative Name: IkappaB-beta

Background: Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further IKBA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.

Molecular Weight: 48 kDa

NCBI Accession: NP_001001

UniProt: Q15653

Pathways: NF-kappaB Signaling, Activation of Innate immune Response, Maintenance of Protein Location, Toll-Like Receptors Cascades