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PI3 Kinase P85 alpha (pTyr467) antibody

Details for Product No. ABIN363134, Supplier: Login to see
Antigen
Epitope
pTyr467
34
18
17
16
13
12
10
7
7
5
4
4
4
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
166
102
81
15
5
Host
Rabbit
157
48
Clonality
Polyclonal
Conjugate
Un-conjugated
7
7
5
5
5
5
2
2
2
2
2
2
2
2
2
Application
Western Blotting (WB)
162
73
71
29
22
22
14
13
10
4
4
1
1
1
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Immunogen Peptide sequence around phosphorylation site of pTyr467 (L-Y (p) -E-E-Y) derived from Human PI3K P85. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of total PI3K P85 only when phosphorylated at tyrosine 467.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name PI3K (p85 alpha)
Background Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1./Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane.
Molecular Weight 85 kDa
UniProt O00459
Pathways
Application Notes Western blotting: 1:500-1:1000
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Neri, Borgatti, Capitani et al.: "The nuclear phosphoinositide 3-kinase/AKT pathway: a new second messenger system." in: Biochimica et biophysica acta, Vol. 1584, Issue 2-3, pp. 73-80, 2002 (PubMed).

Simpson, Parsons: "PTEN: life as a tumor suppressor." in: Experimental cell research, Vol. 264, Issue 1, pp. 29-41, 2001 (PubMed).

Stoyanov, Volinia, Hanck et al.: "Cloning and characterization of a G protein-activated human phosphoinositide-3 kinase." in: Science (New York, N.Y.), Vol. 269, Issue 5224, pp. 690-3, 1995 (PubMed).