CXCL1 antibody

Catalog No. ABIN2621530

This Rabbit Polyclonal antibody specifically detects CXCL1 in WB, ELISA, IHC, Neut and IHC (p). It exhibits reactivity toward Human.

Quick Overview for CXCL1 antibody (ABIN2621530)

Target: CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Neutralization (Neut), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CXCL1 Antibody

Purification: Immunoaffinity purified

Immunogen: Highly pure (>98%) recombinant hGRO/MGSA (human GRO/MGSA).

Application Details

Application Notes: Approved: ELISA (0.2 μg/mL), IHC, IHC-P (5 μg/mL), Neut (1.5 - 3 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hGRO/MGSA 100 ng/mL), a concentration of 1.5 - 3.0 μg/mL of this antibody is required. ELISA: To detect hGRO/MGSA by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hGRO/MGSA. Western Blot: To detect hGRO/MGSA by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGRO/MGSA is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in sterile water

Concentration: Lot specific

Buffer: Lyophilized

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CXCL1

Target: CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))

Alternative Name: GRO / CXCL1

Background: Name/Gene ID: CXCL1
Subfamily: Intercrine alpha
Family: Intercrine

Synonyms: CXCL1, C-X-C motif chemokine 1, Fibroblast secretory protein, FSP, GRO1, GROa, Growth-regulated alpha protein, GRO-alpha(1-73), MGSA, MGSA-a, Small inducible cytokine b1, GRO, MGSA alpha, NAP-3, SCYB1

Gene ID: 2919

UniProt: P09341

Pathways: Autophagy