Integrin alpha M (ITGAM) antibody (Biotin)

Details for Product No. ABIN370845
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

 
Antigen
Synonyms CR3, CR3A, MAC1, Cd11b, Ly-40, Mac-1, Mac-1a, CD11b/CD18, F730045J24Rik, CD11B, MAC-1, MAC1A, MO1A, SLEB6, C3bi
Reactivity
Mouse (Murine)
(315), (249), (63), (22), (19), (16), (13), (7), (5), (3), (2), (1), (1)
Host
Rat
(253), (222), (50), (7), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Biotin
(91), (57), (50), (24), (14), (10), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Flow Cytometry (FACS)
(413), (111), (103), (64), (52), (40), (35), (32), (31), (28), (10), (4), (3), (2), (2), (1), (1), (1)
Pubmed 11 references available
Catalog no. ABIN370845
Quantity 0.5 mg
Price
319.00 $   Plus shipping costs $45.00
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Peritoneal macrophages from C57 B1/6 x DBA/2 F1 hybrid mice. (Ref. 1)
Clone 3A33
Isotype IgG2a
Characteristics Synonyms: Integrin alpha-M, CR3A, MAC-1, MAC1, CR-3 alpha chain, Cell surface glycoprotein MAC-1subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD11antigen-like family member B
Alternative Name CD11b
Background CD11b (Integrin alpha M subunit) combines with CD18 (Integrin beta2 subunit) to form theintegrin Mac-1, also known as complement receptor 3 (CR3), which mediates adhesion toC3bi and ICAM-1 (CD54). (Ref. 2-6) Mac-1 is expressed at varying levels on granulocytes,macrophages, dendritic cells, NK cells, and B-1 cells in the peritoneal and pleural cavities. (Ref. 2,7-10) In addition to its role in binding C3bi on opsonized targets and mediation ofthe subsequent ingestion process. (Ref. 4,11) Mac-1 is important as an adhesion moleculein the transendothelial migration of monocytes and neutrophils. (Ref. 12)
UniProt P05555
Research Area Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Glia marker, CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow Cytometry: < / = 1 µg/10e6 cells. (Ref.1)Immunohistochemistry on Frozen Sections. : (Ref.13)Immunoprecipitation. (Ref.1)Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing Sodium Azide as preservative.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Springer, Galfré, Secher et al.: "Mac-1: a macrophage differentiation antigen identified by monoclonal antibody." in: European journal of immunology, Vol. 9, Issue 4, pp. 301-6, 1979 (PubMed).

Kantor, Stall, Adams et al.: "Differential development of progenitor activity for three B-cell lineages." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 8, pp. 3320-4, 1992 (PubMed).

Vremec, Zorbas, Scollay et al.: "The surface phenotype of dendritic cells purified from mouse thymus and spleen: investigation of the CD8 expression by a subpopulation of dendritic cells." in: The Journal of experimental medicine, Vol. 176, Issue 1, pp. 47-58, 1992 (PubMed).

Gresham, Graham, Anderson et al.: "Leukocyte adhesion-deficient neutrophils fail to amplify phagocytic function in response to stimulation. Evidence for CD11b/CD18-dependent and -independent mechanisms of phagocytosis." in: The Journal of clinical investigation, Vol. 88, Issue 2, pp. 588-97, 1991 (PubMed).

Martin, Le Corre, Pellen et al.: "A monoclonal antibody (3A33) that reacts with a mouse-specific epitope of Mac-1 antigen." in: Tissue antigens, Vol. 28, Issue 1, pp. 15-23, 1986 (PubMed).

Springer, Davignon, Ho et al.: "LFA-1 and Lyt-2,3, molecules associated with T lymphocyte-mediated killing; and Mac-1, an LFA-1 homologue associated with complement receptor function." in: Immunological reviews, Vol. 68, pp. 171-95, 1983 (PubMed).

Sanchez-Madrid, Simon, Thompson et al.: "Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1." in: The Journal of experimental medicine, Vol. 158, Issue 2, pp. 586-602, 1983 (PubMed).

Beller, Springer, Schreiber: "Anti-Mac-1 selectively inhibits the mouse and human type three complement receptor." in: The Journal of experimental medicine, Vol. 156, Issue 4, pp. 1000-9, 1983 (PubMed).

Ault, Springer: "Cross-reaction of a rat-anti-mouse phagocyte-specific monoclonal antibody (anti-Mac-1) with human monocytes and natural killer cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 126, Issue 1, pp. 359-64, 1981 (PubMed).

Leenen, de Bruijn, Voerman et al.: "Markers of mouse macrophage development detected by monoclonal antibodies." in: Journal of immunological methods, Vol. 174, Issue 1-2, pp. 5-19, 1994 (PubMed).

Lub, van Kooyk, Figdor: "Competition between lymphocyte function-associated antigen 1 (CD11a/CD18) and Mac-1 (CD11b/CD18) for binding to intercellular adhesion molecule-1 (CD54)." in: Journal of leukocyte biology, Vol. 59, Issue 5, pp. 648-55, 1996 (PubMed).

Hosts (253), (222), (50), (7), (3)
Reactivities (315), (249), (63), (22), (19), (16), (13), (7), (5), (3), (2), (1), (1)
Applications (413), (111), (103), (64), (52), (40), (35), (32), (31), (28), (10), (4), (3), (2), (2), (1), (1), (1)
Conjugates (91), (57), (50), (24), (14), (10), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (15), (7), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
back to top