CD40 Ligand antibody

Catalog No. ABIN370948

This Hamster Monoclonal antibody specifically detects CD40 Ligand in FACS, IP and Func. It exhibits reactivity toward Mouse.

Quick Overview for CD40 Ligand antibody (ABIN370948)

Target: CD40 Ligand (CD40LG)

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This CD40 Ligand antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func)

Clone: MR1

Product Details anti-CD40 Ligand Antibody

Characteristics: Synonyms: CD40 ligand, CD40-L, CD40LG, TNFSF5, TRAP, GP39, Tumor necrosis factor ligandsuperfamily member 5, TNF-related activation protein, T-cell antigen Gp39

Purification: Purified

Immunogen: Activated mouse Th1 clone D1. 6 (Ref. 1)

Isotype: IgG

Application Details

Application Notes: Flow Cytometry. (Ref.1,5)Immunoprecipitation. Functional Assay: In vivo and in vitro blockage of CD154 function. (Ref.1,3,5)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD40 Ligand

Target: CD40 Ligand (CD40LG)

Alternative Name: CD154 / CD40L

Background: CD154, formerly known as CD40 ligand and gp39, is a type II integral membrane protein and a member of the tumor necrosis factor (TNF) family of ligands. (Ref.1-7) It is an important accessory molecule in T cell-B cell costimulatory interactions, and is expressed predominantly on activated CD4+ T lymphocytes. It is also present on the surface of activated Th0, Th1, and Th2 T cell clones. Its expression is transient and cyclosporinsensitive. (Ref.6)Synonyms: CD40 ligand, CD40-L, CD40LG, GP39, T-cell antigen Gp39, TNF-related activation protein, TNFSF5, TRAP, Tumor necrosis factor ligand superfamily member 5

UniProt: P27548

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints