CD40LG
Reactivity: Human
FACS, ELISA
Host: Mouse
Monoclonal
8H10F5
unconjugated
Application Notes
Flow Cytometry. (Ref.1,5)Immunoprecipitation. Functional Assay: In vivo and in vitro blockage of CD154 function. (Ref.1,3,5) Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
CD154, formerly known as CD40 ligand and gp39, is a type II integral membrane protein and a member of the tumor necrosis factor (TNF) family of ligands. (Ref.1-7) It is an important accessory molecule in T cell-B cell costimulatory interactions, and is expressed predominantly on activated CD4+ T lymphocytes. It is also present on the surface of activated Th0, Th1, and Th2 T cell clones. Its expression is transient and cyclosporinsensitive. (Ref.6)Synonyms: CD40 ligand, CD40-L, CD40LG, GP39, T-cell antigen Gp39, TNF-related activation protein, TNFSF5, TRAP, Tumor necrosis factor ligand superfamily member 5