Add to Basket
Order hotline:
+1 404 474 4654
+1 888 205 9894 (TF)

CD4 Molecule (CD4) antibody

Antigen

CD4 Molecule (CD4)
Synonyms CD4, MGC165891, cd4, CD4mut, L3T4, Ly-4, p55, W3/25
Clonality Monoclonal (EP96)
Host
Alternatives

Mouse
Reactivity
Alternatives

Chicken
Application
Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Functional Studies (Func), Immunoprecipitation (IP)
Catalog no. ABIN371355
Quantity 0.5 mg  (0.5 mg/ml)
Price 290.00 $   Plus shipping costs $35.00
Shipping to
Availability Ships within 7 to 10 Business Days

Additional Information
Application Details Alternatives Secondary Antibodies back to top

Characteristics Synonyms: T4/Leu-3, T cell surface glycoprotein CD4, T Cell Surface Antigen T4/Leu3
Alternative name CD4
Swiss-Prot Q6Q4G0
Cross-Reactivity Chicken
Isotype IgM  (Matching secondary antibodies)
Clone EP96
Description CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to theimmunoglobulin superfamily. CD4 is present on a subset of T lymphocytes(helper/inducer T cells) and is also expressed at a lower level on monocytes, tissuemacrophages and granulocytes. The antigen is involved in binding to MHC class IImolecules. The intracellular domain of the antigen is associated with p56lck proteintyrosine kinase.
Specificity This antibody recognizes Chjicken CD4 (Mr. 64 kDa). EP96 inhibits both PHA- and Con A-induced proliferative responses of splenocytes, andPWM-induced IL-2 production. (Ref. 1)Species: Chicken. Others not tested.

Application Details
Alternatives Secondary Antibodies back to top

Application Notes Flow Cytometry. (Ref.1)Immunohistochemistry (Acetone-Fixed, Frozen Tissue Sections): Identification and localization of CD4+ T cellsImmunoprecipitation. (Ref.1,2)Inhibition of mitogen-induced proliferation (Ref.1,2)Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Concentration 0.5 mg/ml
Purification Purified
Buffer 100 mM Borate Buffered Saline, pH 8.2.No preservatives or amine-containing buffer salts added.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Restrictions For Research Use only

Alternatives
Application Details Secondary Antibodies back to top

Alternatives for antigen "CD4 Molecule (CD4)", type "Antibodies"
Hosts Mouse (444), Rat (208), Rabbit (68)
Reactivities Human (321), Mouse (Murine) (239), Rat (Rattus) (106), Dog (Canine) (44), Cow (Bovine) (34), Chicken (33), Sheep (Ovine) (24), Pig (Porcine) (22), Cat (Feline) (20), Rabbit (20), Bird (Avian) (8), Guinea Pig (8), Horse (Equine) (4), Duck (3), Monkey (3)
Applications Flow Cytometry (FACS) (601), Immunohistochemistry (Frozen Sections) (IHC (fro)) (148), Immunoprecipitation (IP) (92), Western Blotting (WB) (83), Immunofluorescence (IF) (53), ELISA (50), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (49), Functional Studies (Func) (34), Immunohistochemistry (IHC) (29), Immunocytochemistry (ICC) (16), Blocking Antibody (Inhibition) (11), Cytotoxicity Test (CyTox) (11), Enzyme Immunoassay (EIA) (9), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Immunoelectron Microscopy (IEM) (2), Blocking Antibody (Inhibition) (1), ELISA (Detection) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1), Neutralization (Neut) (1)
Conjugates FITC (116), PE (77), Biotin (71), APC (40), RPE (22), Cy5 (9), Cy5,PE (9), Cy5.5 (6), Cy7,PE (6), Alexa Fluor 488 (5), PE,Cy7 (5), HRP (4), PE,Cy5.5 (4), Pacific Blue (4), APC,Cy7 (3), PE,Cy5 (3), PerCP (3), SPRD (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Alexa Fluor 700 (2), Cy3 (2), Cy7 (2), Gold (2), PE,Cy3 (2), RBITC (2), DY647 (1), Dyomics 647 (1), PE,DY590 (1), PE,DY647 (1), PE,Texas Red (TR) (1), PE-Dyomics 590 (1), PE-Dyomics 647 (1), PerCP,Cy5.5 (1), RPE,Cy5 (1), RPE,Cy7 (1)
Epitopes domain 1 (10), domain 2 (5), non-polymorphic (5)