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CD4 Molecule (CD4) antibody

Antigen

CD4 Molecule (CD4)
Synonyms CD4, MGC165891, cd4, CD4mut, L3T4, Ly-4, p55, W3/25
Clonality Monoclonal (RFT-4g)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Application
Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)
Catalog no. ABIN371524
Quantity 0.1 mg  (0.1 mg/ml)  (Variants)
Price 165.00 $   Plus shipping costs $35.00
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Additional Information
Application Details Alternatives Secondary Antibodies Variants back to top

Characteristics Synonyms: T4/Leu-3, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3
Alternative name CD4
Swiss-Prot P01730
Cross-Reactivity Human
Isotype IgG1  (Matching secondary antibodies)
Clone RFT-4g
Description CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to theimmunoglobulin superfamily. CD4 is present on a subset of T lymphocytes(helper/inducer T cells) and is also expressed at a lower level on monocytes, tissuemacrophages and granulocytes. The antigen is involved in binding to MHC class IImolecules. The intracellular domain of the antigen is associated with p56lck proteintyrosine kinase. CD4 functions as an accessary molecule in the recognition of foreign antigens inassociation with MHC Class II antigens by T cells. (Ref. 1-6)
Specificity This antibody recognizes CD4. Species: Human. Others not tested.

Application Details
Alternatives Secondary Antibodies Variants back to top

Application Notes Flow Cytometry: < / = 1 µg/10e6 cells. Immunohistochemistry on Frozen Sections. Immunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Concentration 0.1 mg/ml
Purification Purified
Buffer 100 mM Borate Buffered Saline, pH 8.2.No preservatives or amine-containing buffer salts added.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Restrictions For Research Use only

Alternatives
Application Details Secondary Antibodies Variants back to top

Alternatives for antigen "CD4 Molecule (CD4)", type "Antibodies"
Hosts Mouse (444), Rat (208), Rabbit (68)
Reactivities Human (320), Mouse (Murine) (239), Rat (Rattus) (106), Dog (Canine) (44), Chicken (34), Cow (Bovine) (34), Sheep (Ovine) (24), Pig (Porcine) (22), Cat (Feline) (20), Rabbit (20), Bird (Avian) (8), Guinea Pig (8), Horse (Equine) (4), Duck (3), Monkey (3)
Applications Flow Cytometry (FACS) (601), Immunohistochemistry (Frozen Sections) (IHC (fro)) (148), Immunoprecipitation (IP) (92), Western Blotting (WB) (83), Immunofluorescence (IF) (53), ELISA (50), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (49), Functional Studies (Func) (35), Immunohistochemistry (IHC) (29), Immunocytochemistry (ICC) (16), Blocking Antibody (Inhibition) (11), Cytotoxicity Test (CyTox) (11), Enzyme Immunoassay (EIA) (9), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Immunoelectron Microscopy (IEM) (2), Blocking Antibody (Inhibition) (1), ELISA (Detection) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1), Neutralization (Neut) (1)
Conjugates FITC (116), PE (77), Biotin (71), APC (40), RPE (22), Cy5 (9), Cy5,PE (9), Cy5.5 (6), Cy7,PE (6), Alexa Fluor 488 (5), PE,Cy7 (5), HRP (4), PE,Cy5.5 (4), Pacific Blue (4), APC,Cy7 (3), PE,Cy5 (3), PerCP (3), SPRD (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Alexa Fluor 700 (2), Cy3 (2), Cy7 (2), Gold (2), PE,Cy3 (2), RBITC (2), DY647 (1), Dyomics 647 (1), PE,DY590 (1), PE,DY647 (1), PE,Texas Red (TR) (1), PE-Dyomics 590 (1), PE-Dyomics 647 (1), PerCP,Cy5.5 (1), RPE,Cy5 (1), RPE,Cy7 (1)
Epitopes domain 1 (10), domain 2 (5), non-polymorphic (5)