FBL antibody
| Antigen |
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| Clonality |
Monoclonal (38F3) |
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Host
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Reactivity
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Application
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 Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
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| Catalog no. |
ABIN371824 |
| Quantity |
100 µl |
| Price |
290.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: FIB1, FLRN, Fibrillarin, RNU3IP1, NOP1, LOT3, Nucleolar protein 1, Nucleolar Marker, rRNA2'-O-methyltransferase fibrillarin, U3 small nucleolar RNA-associated protein NOP1
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Gene ID
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851548
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Swiss-Prot
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P15646
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Immunogen
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Yeast nuclear preparations.
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Cross-Reactivity
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Caenorhabditis elegans (C. elegans), Fruit Fly (Drosophila melanogaster), Human, Rat (Rattus), Schizosaccharomyces pombe (S. pombe)
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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38F3
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Description
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Nop1p was originally identified as a nucleolar protein of bakers yeast, Saccharomycescerevisiae. The Nop1p protein is 327 amino acids in size (34. 5kDa), is essential for yeastviability, and is localized in the nucleoli (1). The systematic name for S. cerevisiae Nop1 isYDL014W, and it is now known to be part of the small subunit processome complex,involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of aprotein found in all eukaryotes and archea generally called fibrillarin (2). Fibrillarin/Nop1pis extraordinarily conserved, so that the yeast and human proteins are 67% identical, andthe human protein can functionally replace the yeast protein. Patients with theautoimmune disease scleroderma often have strong circulating autoantibodies to a~34kDa protein which was subsequently found to be fibrillarin. Recent studies show thatknock-out of the fibrillarin gene in mice results in embryonic lethality, although mice withonly one functional fibrillarin/Nop1p gene were viable (3).
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Specificity
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Specific for the ~34kDa Fibrillarin /Nop1p protein. This antibody is becoming widely used as a convenient marker for nucleoli in a wide varietyof species (e. g. 4-6). Species Reactivity: Tested: Human, Rat, Plant, Drosophila, C. elegans and S. pombe. Expected from sequence similarity: Mammals.
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Application Notes
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Western Blot: 1/1,000. Immunofluorescence: 1/500. Immunohistochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Purification
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Ig Fraction
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Storage
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Store the antibody undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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Restrictions
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For Research Use only
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Alternatives for antigen "FBL", type "Antibodies"
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Hosts
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Mouse (12), Rabbit (1)
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Reactivities
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Human (8), Yeast (Pichia pastoris) (3), Fruit Fly (Drosophila melanogaster) (1), Mammalian (1), Yeast (1)
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Applications
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Western Blotting (WB) (9), Immunofluorescence (IF) (5), Enzyme Immunoassay (EIA) (4), Immunoprecipitation (IP) (3), ELISA (2), Dot Blot (Dot) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (1)
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Epitopes
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Center (1)
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