HSD11B2 antibody (AA 25-40)

Catalog No. ABIN372479

The Rabbit Polyclonal anti-HSD11B2 antibody is suitable to detect HSD11B2 in samples from Human, Mouse and Rat. It has been validated for WB and IHC (p).

Quick Overview for HSD11B2 antibody (AA 25-40) (ABIN372479)

Target: HSD11B2 (Hydroxysteroid (11-Beta) Dehydrogenase 2 (HSD11B2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSD11B2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HSD11B2 Antibody

Binding Specificity: AA 25-40

Sequence: R25SDLRLGRPLL AALAL4

Specificity: This antibody recognizes 11-HSD2.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide corresponding to amino acids 25-40 of human 11 beta-HSD2.

Isotype: IgG

Application Details

Application Notes: Western blot: 1/200. Immunohistochemistry on Paraffin Sections: 10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: TBS, pH 7.4 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA, 50 % Glycerol as stabilizers.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for HSD11B2

Target: HSD11B2 (Hydroxysteroid (11-Beta) Dehydrogenase 2 (HSD11B2))

Alternative Name: 11-beta HSD2 / HSD11B2

Background: HSD11B2 catalyzes the conversion of cortisol to the inactive metabolite cortisone. The protein modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids. Defects in HSD11B2 are the cause of apparent mineralocorticoid excess (AME). AME is a potentially fatal disease characterized by severe juvenile low-renin hypertension, sodium retention, hypokalemia and low levels of aldosterone. It often leads to nephrocalcinosis.Synonyms: 11-DH2, 11-beta-hydroxysteroid dehydrogenase 2, Corticosteroid 11-beta-dehydrogenase isozyme 2, HSD11K, NAD-dependent 11-beta-hydroxysteroid dehydrogenase

Gene ID: 3291

NCBI Accession: NP_000187

UniProt: P80365

Pathways: Steroid Hormone Biosynthesis, Regulation of Systemic Arterial Blood Pressure by Hormones