APP antibody (C-Term)

Catalog No. ABIN372589

This Rabbit Polyclonal antibody specifically detects APP in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Chicken and Non-Human Primate.

Quick Overview for APP antibody (C-Term) (ABIN372589)

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Reactivity: Human, Mouse, Rat, Dog, Cow, Chicken, Non-Human Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APP antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-APP Antibody

Binding Specificity: C-Term

Specificity: Specific for ~115k APP protein. Immunolabeling of the APP protein band is completely blocked by preadsorption of the antibody with the peptide used as antigen.

Cross-Reactivity (Details): Species reactivity (expected):Human, Canine, Chicken, Mouse and non-Human Primates.
Species reactivity (tested):Rat.

Purification: Affinity Chromatography.

Immunogen: Peptide corresponding to amino acid residues from the C-terminal region of rat APP.

Isotype: IgG

Application Details

Application Notes: Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

Target Details for APP

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Alternative Name: Amyloid beta A4 Protein / APP

Background: A large body of evidence has implicated the amyloid precursor protein (APP) in the pathogenesis of Alzheimer's disease (AD) (Kamenetz et al., 2003). The phosphorylation of APP at Thr668 is thought to play a critical role in generation of the soluble APP (beta) and beta-amyloid peptide (abeta) which are the major components of senile plaques in patient brains inflicted with AD (Liu et al., 2003, Ando et al., 2001).Synonyms: ABPP, APPI, Alzheimer disease amyloid protein, Amyloid Precursor Protein, CVAP, Cerebral vascular amyloid peptide, PreA4, Protease nexin-II

Gene ID: 54226

NCBI Accession: NP_062161

UniProt: P08592

Pathways: Caspase Cascade in Apoptosis, EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, Feeding Behaviour