CAMP Responsive Element Binding Protein 1 (CREB1) (pSer133) antibody

Details for Product No. ABIN372604
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Antigen
Synonyms creb, CREB1, Creb1, CREB, 2310001E10Rik, 3526402H21Rik, AV083133, Creb, Creb-1, creb1, zgc:55598
Epitope
pSer133
(81), (37), (30), (20), (20), (16), (15), (9), (8), (7), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Non Human Primates, Rat (Rattus), Sheep (Ovine), Xenopus laevis, Zebrafish (Danio rerio)
(389), (244), (231), (75), (67), (66), (44), (8), (4), (4), (3), (1), (1), (1), (1)
Host
Rabbit
(348), (57), (2), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(12), (10), (10), (7), (6), (6), (6), (6), (6), (6), (6), (2), (2), (2)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(309), (166), (151), (74), (60), (50), (31), (25), (22), (15), (11), (4), (2), (2), (2), (2), (1), (1)
Pubmed 6 references available
Quantity 0.1 mL
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Catalog No. ABIN372604
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Immunogen Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser133 of rat CREB.
Isotype IgG
Specificity Specific for the ~45k CREB protein phosphorylated at Ser133. Immunolabeling is blocked by lambda-phosphatase treatment.
Cross-Reactivity (Details) Species reactivity (expected):Bovine, Canine, Chicken, Human, Mouse, Sheep, Xenopus, Zebrafish and non-Human Primates.
Species reactivity (tested):Rat.
Purification Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
Alternative Name CREB1
Background It is well known that the control of gene expression involves activation of protein kinase cascades that regulate transcription factors within the nucleus (Karin and Hunter, 1995). The cyclic AMP response element binding protein (CREB) is one of the best characterized stimulus-induced transcription factors (Montminy, 1997). This transcription factor is a component of intracellular signaling events that regulate a wide range of biological functions, from spermatogenesis to circadian rhythms and memory (Shaywitz and Greenberg, 1999, Silva et al., 1998). A variety of protein kinases including protein kinase A (PKA), mitogen-activated protein kinases (MAPKs), and Ca2+/calmodulin-dependent protein kinases (CaMKs) phosphorylate CREB at serine 133 (Ser133), and phosphorylation of Ser133 are required for CREB-mediated transcription (Johannessen et al., 2004, Kornhauser et al., 2002).Synonyms: CREB-1, Cyclic AMP-responsive element-binding protein 1, cAMP-responsive element-binding protein 1
Gene ID 81646
NCBI Accession NP_112279
UniProt P15337
Application Notes Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at-20 °C.
Supplier Images
anti-CAMP Responsive Element Binding Protein 1 (CREB1) (pSer133) antibody anti-CAMP Responsive Element Binding Protein 1 (CREB1) (pSer133) antibody
Background publications Karin, Hunter: "Transcriptional control by protein phosphorylation: signal transmission from the cell surface to the nucleus." in: Current biology : CB, Vol. 5, Issue 7, pp. 747-57, 1995 (PubMed).

Montminy: "Transcriptional regulation by cyclic AMP." in: Annual review of biochemistry, Vol. 66, pp. 807-22, 1997 (PubMed).

Silva, Kogan, Frankland et al.: "CREB and memory." in: Annual review of neuroscience, Vol. 21, pp. 127-48, 1998 (PubMed).

Shaywitz, Greenberg: "CREB: a stimulus-induced transcription factor activated by a diverse array of extracellular signals." in: Annual review of biochemistry, Vol. 68, Issue 2, pp. 821-61, 2000 (PubMed).

Kornhauser, Cowan, Shaywitz et al.: "CREB transcriptional activity in neurons is regulated by multiple, calcium-specific phosphorylation events." in: Neuron, Vol. 34, Issue 2, pp. 221-33, 2002 (PubMed).

Johannessen, Delghandi, Moens: "What turns CREB on?" in: Cellular signalling, Vol. 16, Issue 11, pp. 1211-27, 2004 (PubMed).

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