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CREB1 antibody (CAMP Responsive Element Binding Protein 1) (pSer133)

Details for Product anti-CREB1 Antibody No. ABIN372604, Supplier: Login to see
Antigen
  • creb
  • CREB1
  • Creb1
  • CREB
  • 2310001E10Rik
  • 3526402H21Rik
  • AV083133
  • Creb
  • Creb-1
  • creb1
  • zgc:55598
Alternatives
anti-Human CREB1 antibody for Western Blotting
Epitope
pSer133
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36
33
28
22
22
17
17
16
13
10
7
7
5
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4
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3
3
3
3
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3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Non Human Primates, Rat (Rattus), Sheep (Ovine), Xenopus laevis, Zebrafish (Danio rerio)
468
223
219
22
20
16
13
12
9
8
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5
2
1
1
Host
Rabbit
404
70
10
1
Clonality
Polyclonal
Conjugate
This CREB1 antibody is un-conjugated
15
14
13
7
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6
6
5
4
3
3
3
3
3
3
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
367
216
183
64
62
39
34
29
25
11
7
3
3
2
1
1
1
1
1
Supplier
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Immunogen Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser133 of rat CREB.
Isotype IgG
Specificity Specific for the ~45k CREB protein phosphorylated at Ser133. Immunolabeling is blocked by lambda-phosphatase treatment.
Cross-Reactivity (Details) Species reactivity (expected):Bovine, Canine, Chicken, Human, Mouse, Sheep, Xenopus, Zebrafish and non-Human Primates.
Species reactivity (tested):Rat.
Purification Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
Alternative Name CREB1 (CREB1 Antibody Abstract)
Background It is well known that the control of gene expression involves activation of protein kinase cascades that regulate transcription factors within the nucleus (Karin and Hunter, 1995). The cyclic AMP response element binding protein (CREB) is one of the best characterized stimulus-induced transcription factors (Montminy, 1997). This transcription factor is a component of intracellular signaling events that regulate a wide range of biological functions, from spermatogenesis to circadian rhythms and memory (Shaywitz and Greenberg, 1999, Silva et al., 1998). A variety of protein kinases including protein kinase A (PKA), mitogen-activated protein kinases (MAPKs), and Ca2+/calmodulin-dependent protein kinases (CaMKs) phosphorylate CREB at serine 133 (Ser133), and phosphorylation of Ser133 are required for CREB-mediated transcription (Johannessen et al., 2004, Kornhauser et al., 2002).Synonyms: CREB-1, Cyclic AMP-responsive element-binding protein 1, cAMP-responsive element-binding protein 1
Gene ID 81646
NCBI Accession NP_112279
UniProt P15337
Pathways TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at-20 °C.
Supplier Images
 image for anti-CREB1 antibody (CAMP Responsive Element Binding Protein 1) (pSer133) (ABIN372604) anti-CAMP Responsive Element Binding Protein 1 (CREB1) (pSer133) antibody
Background publications Johannessen, Delghandi, Moens: "What turns CREB on?" in: Cellular signalling, Vol. 16, Issue 11, pp. 1211-27, 2004 (PubMed).

Kornhauser, Cowan, Shaywitz et al.: "CREB transcriptional activity in neurons is regulated by multiple, calcium-specific phosphorylation events." in: Neuron, Vol. 34, Issue 2, pp. 221-33, 2002 (PubMed).

Shaywitz, Greenberg: "CREB: a stimulus-induced transcription factor activated by a diverse array of extracellular signals." in: Annual review of biochemistry, Vol. 68, Issue 2, pp. 821-61, 2000 (PubMed).

Silva, Kogan, Frankland et al.: "CREB and memory." in: Annual review of neuroscience, Vol. 21, pp. 127-48, 1998 (PubMed).

Montminy: "Transcriptional regulation by cyclic AMP." in: Annual review of biochemistry, Vol. 66, pp. 807-22, 1997 (PubMed).

Karin, Hunter: "Transcriptional control by protein phosphorylation: signal transmission from the cell surface to the nucleus." in: Current biology : CB, Vol. 5, Issue 7, pp. 747-57, 1995 (PubMed).