ELK1 antibody (pSer383)

Catalog No. ABIN372618

The Rabbit Polyclonal anti-ELK1 antibody (ABIN372618) specifically detects ELK1 in WB. The antibody is reactive with Human, Mouse, Rat, Dog, Zebrafish (Danio rerio) and Non-Human Primate samples.

Quick Overview for ELK1 antibody (pSer383) (ABIN372618)

Target: ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Non-Human Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ELK1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ELK1 Antibody

Binding Specificity: pSer383

Specificity: Specific for ~46k Elk-1 phosphorylated at Ser383. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.

Cross-Reactivity (Details): Species reactivity (expected):Human, Canine, Mouse, Zebrafish and non-Human Primates.
Species reactivity (tested):Rat.

Purification: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser383 of human Elk-1.

Isotype: IgG

Application Details

Application Notes: Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

Target Details for ELK1

Target: ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))

Alternative Name: ELK1

Background: Elk-1 is a transcription factor involved in mediating gene transcription in response to growth factors (Hill and Treisman, 1995). Elk-1 is thought to be phosphorylated by MAP kinase at Ser383 and phosphorylation at this site is essential for the transcriptional activity of Elk-1 (Li et al., 2003). Phosphorylation of Elk-1 has also been implicated in synaptic plasticity in the adult hippocampus (Thiels et al., 2002).Synonyms: ETS domain-containing protein Elk-1, Elk-1

Gene ID: 2002

NCBI Accession: NP_001107595

UniProt: P19419

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling