GABBR2 antibody (pSer783)

Catalog No. ABIN372643

This Rabbit Polyclonal antibody specifically detects GABBR2 in WB and IF. It exhibits reactivity toward Human, Rat, Mouse, Cow, Dog, Chicken and Non-Human Primate.

Quick Overview for GABBR2 antibody (pSer783) (ABIN372643)

Target: GABBR2 (gamma-aminobutyric Acid (GABA) B Receptor, 2 (GABBR2))

Reactivity: Human, Rat, Mouse, Cow, Dog, Chicken, Non-Human Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABBR2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-GABBR2 Antibody

Binding Specificity: pSer783

Specificity: Specific for ~102k GABAB R2 phosphorylated at Ser783. Immunolabeling of the GABAB R2 band is completely blocked by lambda-phosphatase treatment.

Cross-Reactivity (Details): Species reactivity (expected):Human, Bovine, Chicken, Mouse, Xenopus and non-Human Primates.
Species reactivity (tested):Rat.

Purification: Sequential Chromatography on phospho- and dephospho-peptide affinity columns.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser783 of GABAB R2.

Isotype: IgG

Application Details

Application Notes: Western Blot: 1/1000. Immunofluorescence: 1/500 (See Ref.1)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

Target Details for GABBR2

Target: GABBR2 (gamma-aminobutyric Acid (GABA) B Receptor, 2 (GABBR2))

Alternative Name: GABBR2

Background: Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAB receptors are heterodimeric G protein-coupled receptors that mediate slow synaptic inhibition in the central nervous system. It has recently been demonstrated that AMPK binds directly to GABAB receptors and phosphorylates S783 in the cytoplasmic tail of the R2 subunit and that S783 plays a critical role in enhancing neuronal survival after ischemia as phosphorylation of S783 is evident in many brain regions and is increased dramatically after ischemic injury to the brain (Kuramoto et al., 2007).Synonyms: G-protein coupled receptor 51, GABA-B receptor 2, GABA-B-R2, GABBR-2, GPR51, GPRC3B, Gamma-aminobutyric acid type B receptor subunit 2, Gb2, HG20

Gene ID: 83633

NCBI Accession: NP_113990

UniProt: O88871

Pathways: cAMP Metabolic Process