PC antibody (Internal Region)

Catalog No. ABIN372799

Product Details anti-PC Antibody

Target: PC (Pyruvate Carboxylase (PC))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Pig, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This PC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: Recognizes Pyruvate Carboxylase (PC).

Cross-Reactivity (Details): Species reactivity (expected):Bovine, Canine (Dog), Mouse, Porcine and Rat.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide (C-KFKEVKKAYVEANQ) from an Internal region of Human PC.

Isotype: IgG

Application Details

Application Notes: ELISA: 1/16000. Western blot: 0.03-0.1 μg/mL. Immunohistochemistry on Paraffin Sections: 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: Tris saline buffer, pH 7.3 containing 0.5 % BSA as stabilizer and 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for PC

Target: PC (Pyruvate Carboxylase (PC))

Alternative Name: Pyruvic Carboxylase (PC) (PC Products)

Synonyms: BcDNA antibody, BcDNA:GH06348 antibody, Dmel\\CG1516 antibody, PC antibody, pyc antibody, wu:fb82g08 antibody, wu:fj85g01 antibody, PCX antibody, BA4157 antibody, Pc antibody, Pcb antibody, PCB antibody, Pcx antibody, Pyruvate carboxylase antibody, pyruvate carboxylase b antibody, PYruvate Carboxylase antibody, pyruvate carboxylase antibody, pyruvate carboxylase Pyr1 antibody, PCB antibody, pcxb antibody, pyc-1 antibody, PC antibody, pyc antibody, pyr1 antibody, CNF00650 antibody, PYC1 antibody, Ndas_1152 antibody, Ilyop_2503 antibody, Calni_0495 antibody, Ftrac_1004 antibody, Plabr_3574 antibody, Sgly_2936 antibody, Celly_0558 antibody, Hipma_0131 antibody, Halhy_1567 antibody, Mesop_1269 antibody, Pcx antibody, Pc antibody

Background: Pyruvate Carboxylase (PC, PCB) requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. PCB is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene.Synonyms: PCB, Pyruvate carboxylase mitochondrial

Gene ID: 5091

NCBI Accession: NP_000911

UniProt: P11498