SOCS3 antibody (C-Term)

Catalog No. ABIN374066

The Goat Polyclonal anti-SOCS3 antibody has been validated for WB. It is suitable to detect SOCS3 in samples from Human, Mouse, Rat, Cow, Pig and Dog.

Quick Overview for SOCS3 antibody (C-Term) (ABIN374066)

Target: SOCS3 (Suppressor of Cytokine Signaling 3 (SOCS3))

Reactivity: Human, Mouse, Rat, Cow, Pig, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This SOCS3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SOCS3 Antibody

Binding Specificity: C-Term

Sequence: C-PIREFLDQYD APL

Specificity: This antibody detects SOCS3 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, canine (Dog), Pig, Cow.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_003946.3. Genename: SOCS3

Application Details

Application Notes: ELISA: Limit dilution: 1/16000. Western Blot: 4-6 μg/mL. Approx 24 kDa band observed in MOLT-4 lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide and 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SOCS3

Target: SOCS3 (Suppressor of Cytokine Signaling 3 (SOCS3))

Alternative Name: SOCS3 / CIS3

Background: Accumulating evidence demonstrates that cytokine receptor signaling is negatively regulated by a family of Src homology 2 domain-containing adaptor molecules Termed SOCS (Suppressor of Cytokine Signaling). To date, there are eight members of SOCS family have been recognized, they are SOCS-1, 2, 3, 4, 5, 6, 7 and CIS. Structurally the SOCS proteins are composed of an N-terminal region of variable length and amino acid composition, a central SH2 domain, and a previously unrecognized C-terminal motif that we have called the SOCS box. The SOCS proteins appear to form part of a classical negative feed back loop that regulates cytokine signal transduction via a STAT-induced transcriptional mechanism. Transcription of each of the SOCS genes occurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signaling in different ways.Synonyms: CIS-3, Cytokine-inducible SH2 protein 3, SOCS-3, SSI-3, SSI3, STAT-induced STAT inhibitor 3, Suppressor of cytokine signaling 3

Gene ID: 9021

NCBI Accession: NP_003946

UniProt: O14543

Pathways: JAK-STAT Signaling, Response to Growth Hormone Stimulus, Hepatitis C