SOCS3 antibody (Middle Region)

Catalog No. ABIN3044272

This Rabbit Polyclonal antibody specifically detects SOCS3 in WB and IHC. It exhibits reactivity toward Human and Mouse. It has been mentioned in 2+ publications

Quick Overview for SOCS3 antibody (Middle Region) (ABIN3044272)

Target: SOCS3 (Suppressor of Cytokine Signaling 3 (SOCS3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOCS3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SOCS3 Antibody

Binding Specificity: AA 71-85, Middle Region

Purpose: Anti-SOCS3 Antibody

Sequence: RDSSDQRHFF TLSVK

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-SOCS3 Antibody (ABIN3044272). Tested in IHC, WB applications. This antibody reacts with Human, Mouse. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human SOCS3, identical to the related mouse sequence, different from the related rat sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse
Western blot, 0.1-0.5 μg/mL, Human, Mouse

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-SOCS3 antibody (ABIN3044272)

Yang, Tian, Zhang, Xu, Yang, Yu, Li, Li, Li, Li: "Role of the JAK2/STAT3 signaling pathway in the pathogenesis of type 2 diabetes mellitus with macrovascular complications." in: Oncotarget, Vol. 8, Issue 57, pp. 96958-96969, (2017) (PubMed).

Wan, Che, Kang, Wu: "SOCS3 blocks HIF-1? expression to inhibit proliferation and angiogenesis of human small cell lung cancer by downregulating activation of Akt, but not STAT3." in: Molecular medicine reports, Vol. 12, Issue 1, pp. 83-92, (2015) (PubMed).

Target Details for SOCS3

Target: SOCS3 (Suppressor of Cytokine Signaling 3 (SOCS3))

Alternative Name: SOCS3

Background:

Synonyms: Suppressor of cytokine signaling 3,SOCS-3,Cytokine-inducible SH2 protein 3,CIS-3,STAT-induced STAT inhibitor 3,SSI-3,SOCS3,CIS3, SSI3,

Tissue Specificity: Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus.

Background: SOCS3 (Suppressor of cytokine signaling 3) is a protein that in humans is encoded by the SOCS3 gene. SOCS3 is transiently expressed by multiple cell lineages within the immune system and functions predominantly as a negative regulator of cytokines that activate the JAK-STAT3 pathway. This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene is induced by various cytokines, including IL6, IL10, and interferon (IFN)-gamma. The protein encoded by this gene can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. For signaling of IL-6, Epo, GCSF and Leptin, binding of SOCS3 to the respective cytokine receptor has been found to be crucial for the inhibitory function of SOCS3. Studies of the mouse counterpart of this gene suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis, and placental development.

Sequence Similarities: Contains 1 SH2 domain.

Molecular Weight: 30 kDa

UniProt: O14543

Pathways: JAK-STAT Signaling, Response to Growth Hormone Stimulus, Hepatitis C