SOCS3 antibody (Middle Region)

Catalog No. ABIN3044272

Product Details anti-SOCS3 Antibody

Target: SOCS3 (Suppressor of Cytokine Signaling 3 (SOCS3))

Binding Specificity: AA 71-85, Middle Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOCS3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Suppressor of cytokine signaling 3(SOCS3) detection. Tested with WB, IHC-P in Human,Mouse.

Sequence: RDSSDQRHFF TLSVK

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Suppressor of cytokine signaling 3(SOCS3) detection. Tested with WB, IHC-P in Human,Mouse.
Gene Name: suppressor of cytokine signaling 3
Protein Name: Suppressor of cytokine signaling 3(SOCS-3)

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human SOCS3(71-85aa RDSSDQRHFFTLSVK), identical to the related mouse sequence, different from the related rat sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-SOCS3 antibody (ABIN3044272)

Yang, Tian, Zhang, Xu, Yang, Yu, Li, Li, Li, Li: "Role of the JAK2/STAT3 signaling pathway in the pathogenesis of type 2 diabetes mellitus with macrovascular complications." in: Oncotarget, Vol. 8, Issue 57, pp. 96958-96969, (2017) (PubMed).

Wan, Che, Kang, Wu: "SOCS3 blocks HIF-1? expression to inhibit proliferation and angiogenesis of human small cell lung cancer by downregulating activation of Akt, but not STAT3." in: Molecular medicine reports, Vol. 12, Issue 1, pp. 83-92, (2015) (PubMed).

Target Details for SOCS3

Target: SOCS3 (Suppressor of Cytokine Signaling 3 (SOCS3))

Alternative Name: SOCS3 (SOCS3 Products)

Synonyms: socs3 antibody, fj09e12 antibody, zgc:56537 antibody, wu:fj09e12 antibody, MGC64393 antibody, MGC75749 antibody, xsocs3 antibody, MGC81248 antibody, atod4 antibody, cis3 antibody, cish3 antibody, socs-3 antibody, ssi-3 antibody, ssi3 antibody, SOCS3 antibody, ATOD4 antibody, CIS3 antibody, Cish3 antibody, SOCS-3 antibody, SSI-3 antibody, SSI3 antibody, Cis3 antibody, EF-10 antibody, Ef10 antibody, Ssi3 antibody, Socs-3 antibody, Ssi-3 antibody, suppressor of cytokine signaling 3a antibody, suppressor of cytokine signaling 3 S homeolog antibody, suppressor of cytokine signaling 3 antibody, suppressor of cytokine signaling 3 L homeolog antibody, socs3a antibody, socs3.S antibody, socs3 antibody, socs3.L antibody, SOCS3 antibody, Socs3 antibody

Background: SOCS3(Suppressor of cytokine signaling 3) is a protein that in humans is encoded by the SOCS3 gene. SOCS3 is transiently expressed by multiple cell lineages within the immune system and functions predominantly as a negative regulator of cytokines that activate the JAK-STAT3 pathway. This gene encodes a member of the STAT-induced STAT inhibitor(SSI), also known as suppressor of cytokine signaling(SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene is induced by various cytokines, including IL6, IL10, and interferon(IFN)-gamma. The protein encoded by this gene can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. For signaling of IL-6, Epo, GCSF and Leptin, binding of SOCS3 to the respective cytokine receptor has been found to be crucial for the inhibitory function of SOCS3. Studies of the mouse counterpart of this gene suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis, and placental development.

Synonyms: ATOD4 antibody|CIS 3 antibody|CIS-3 antibody|CIS3 antibody|Cish3 antibody|Cytokine induced SH2 protein 3 antibody|Cytokine-inducible SH2 protein 3 antibody|E2a Pbx1 target gene in fibroblasts 10 antibody|EF 10 antibody|MGC71791 antibody|SOCS 3 antibody|SOCS-3 antibody|Socs3 antibody|SOCS3_HUMAN antibody|SSI 3 antibody|SSI-3 antibody|SSI3 antibody|STAT induced STAT inhibitor 3 antibody|STAT-induced STAT inhibitor 3 antibody|Suppressor of cytokine signaling 3 antibody

UniProt: O14543

Pathways: JAK-STAT Signaling, Response to Growth Hormone Stimulus, Hepatitis C