TRPM7 antibody (C-Term)

Catalog No. ABIN374098

This Goat Polyclonal antibody specifically detects TRPM7 in IHC (p) and EIA. It exhibits reactivity toward Human, Mouse, Rat, Pig and Dog.

Quick Overview for TRPM7 antibody (C-Term) (ABIN374098)

Target: TRPM7 (Transient Receptor Potential Cation Channel, Subfamily M, Member 7 (TRPM7))

Reactivity: Human, Mouse, Rat, Pig, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This TRPM7 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-TRPM7 Antibody

Binding Specificity: C-Term

Sequence: C-TKESESTNSV RLML

Specificity: This antibody detects TRPM7 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Human, Canine (Dog) and Pig.
Species reactivity (tested):Mouse, Rat, Crayfish.

Purification: Ammonium sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from C-Terminus of the protein sequence according to NP_060142.3. Genename: TRPM7

Application Details

Application Notes: Peptide ELISA: 1/16000. Western blot: 0.3-1.0 μg/mL. Approx.150 kDa band observed in Human Brain (Cerebellum)lysates (calculated MW of 210 kDa according to NP_060142.2). The observed molecularweight corresponds to earlier findings in literature with different antibodies. (Ref.3)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for TRPM7

Target: TRPM7 (Transient Receptor Potential Cation Channel, Subfamily M, Member 7 (TRPM7))

Alternative Name: TRPM7

Background: The TRPM7 (Transient Receptor Protein-PhosphoLipase C-Interacting Kinase) protein is a member of the ion channel proteins. It regulates the calcium concentration in cells, which is imperative for such functions as muscle contractions and neuronal firing. TRPM7, however, is unique among the TRP channel proteins because it is bi-functional. It not only controls ion channels but it also acts as a kinase, phosphorylating proteins.Synonyms: CHAK1, Channel-kinase 1, LTRPC7, Long transient receptor potential channel 7, Transient receptor potential cation channel subfamily M member 7

Gene ID: 54822

NCBI Accession: NP_060142

UniProt: Q96QT4